Abstract：
Microsporidia is a diverse group of obligate, intracellular, and spore-forming parasites that infect a wide range of animals. Among them, Enterocytozoon bieneusi and Encephalitozoon spp. are the most frequently reported species in humans. Limited information is available about the presence and molecular diversity of microsporidian species in the endangered Iberian lynx (Lynx pardinus). Presence of Enterocytozoon bieneusi and Encephalitozoon spp. was investigated by molecular methods in wild and captive Iberian lynxes from Spain. Overall, E. bieneusi was detected in 3.2% (8/251) of the animals examined. None of the samples tested were positive for Encephalitozoon spp. Four known (D, EbfelA, PigEBITS7, and Type IV) and a novel (named as LynxSpEb1) E. bieneusi genotypes were identified. All the genotypes found belonged to the zoonotic Group 1 of E. bieneusi. This study provides the first genotyping data of E. bieneusi in Iberian lynx in Spain. Our result indicate that the Iberian lynx does not seem to play a relevant role in the epidemiology of Encephalitozoon spp., and that this endangered felid is likely acting as spillover host rather than a true reservoir of E. bieneusi. Additional studies should be conducted to assess the impact of this parasite in the health status of the endangered Iberian lynx.
摘要：
微孢子虫是一个多样化的专性群体，感染多种动物的细胞内和孢子形成寄生虫。其中,肠孢子虫和头孢菌素。是人类中最常见的物种。关于濒临灭绝的伊比利亚山猫（Lynxpardinus）中微孢子虫物种的存在和分子多样性的信息有限。肠孢子虫和头孢菌素的存在。通过分子方法对来自西班牙的野生和圈养的伊比利亚lynxes进行了研究。总的来说,在3.2%(8/251)的受检动物中检测到E.bieneusi。所测试的样品均未对头孢菌素属阳性。四个已知（D，EbfelA,鉴定了PigEBITS7和IV型）和一种新的（称为LynxSpEb1）E.bieneusi基因型。发现的所有基因型均属于E.bieneusi的人畜共患组1。这项研究提供了西班牙伊比利亚山猫的E.bieneusi的第一个基因分型数据。我们的结果表明，伊比利亚山猫似乎在头孢菌素的流行病学中没有发挥相关作用。,这种濒临灭绝的猫科动物很可能是溢出宿主，而不是真正的E.bieneusi水库。应进行其他研究以评估这种寄生虫对濒危伊比利亚猫的健康状况的影响。
在濒临灭绝的伊比利亚山猫的自由放养和圈养种群中研究了人畜共患的微孢子虫物种。我们的结果表明，该物种可以被认为是肠孢子虫的溢出宿主，并提供了新的E.bieneusi基因型的证据。
在濒临灭绝的伊比利亚山猫的自由放养和圈养种群中研究了人畜共患的微孢子虫物种。我们的结果表明，该物种可以被认为是肠孢子虫的溢出宿主，并提供了新的E.bieneusi基因型的证据。
