PDF(Pubmed)
Abstract：
Pepper (Capsicum annuum L.) is a herbaceous plant species in the family Solanaceae. Capsicum anthracnose is caused by the genus Colletotrichum. spp., which decreases pepper production by about 50% each year due to anthracnose. In this study, we evaluated the resistance of red ripe fruits from 17 pepper varieties against anthracnose fungus Colletotrichum capsici. We assessed the size of the lesion diameter and conducted significance analysis to identify the resistant variety of B158 and susceptible variety of B161. We selected a resistant cultivar B158 and a susceptible cultivar B161 of pepper and used a transcription to investigate the molecular mechanisms underlying the plant\'s resistance to C. capsici, of which little is known. The inoculated fruit from these two varieties were used for the comparative transcription analysis, which revealed the anthracnose-induced differential transcription in the resistant and susceptible pepper samples. In the environment of an anthrax infection, we found that there were more differentially expressed genes in resistant varieties compared to susceptible varieties. Moreover, the response to stimulus and stress ability was stronger in the KANG. The transcription analysis revealed the activation of plant hormone signaling pathways, phenylpropanoid synthesis, and metabolic processes in the defense response of peppers against anthracnose. In addition, ARR-B, AP2-EREBP, bHLH, WRKY, and NAC are associated with disease resistance to anthracnose. Notably, WRKY and NAC were found to have a potentially positive regulatory role in the defense response against anthracnose. These findings contribute to a more comprehensive understanding of the resistance mechanisms of red pepper fruit to anthracnose infection, providing valuable molecular insights for further research on the resistance mechanisms and genetic regulations during this developmental stage of pepper.
摘要：
辣椒（CapsicumannuumL.）是茄科中的一种草本植物。辣椒炭疽病是由炭疽病属引起的。spp.,由于炭疽病，辣椒产量每年减少约50%。在这项研究中,我们评估了17个辣椒品种的红色成熟果实对炭疽病真菌辣椒炭疽病的抗性。我们评估了病变直径的大小，并进行了显著性分析，以鉴定B158的抗性品种和B161的易感品种。我们选择了辣椒的抗性品种B158和易感品种B161，并使用转录来研究植物对辣椒的抗性的分子机制。其中鲜为人知。将这两个品种的接种果实用于比较转录分析，揭示了抗性和易感辣椒样品中炭疽病诱导的差异转录。在炭疽感染的环境中，我们发现，与易感品种相比，抗性品种中差异表达基因更多。此外，KANG对刺激和压力的反应能力更强。转录分析揭示了植物激素信号通路的激活,苯丙素合成，和辣椒对炭疽病的防御反应中的代谢过程。此外,ARR-B,AP2-EREBP,bHLH,WRKY,和NAC与炭疽病的抗病性有关。值得注意的是,发现WRKY和NAC在针对炭疽病的防御反应中具有潜在的积极调节作用。这些发现有助于更全面地了解红辣椒果实对炭疽病感染的抗性机制。为进一步研究辣椒这一发育阶段的抗性机制和遗传调控提供有价值的分子见解。
