PDF(Pubmed)
Abstract：
Rift Valley Fever (RVF) is a mosquito-borne viral zoonosis caused by RVFV in humans and livestock. Currently, there are no approved vaccines or antiviral therapies available. Additionally, in Saudi Arabia, there is a lack of a routine screening system to monitor RVFV in humans and animals which hinders to design and develop the preventive measures as well as the prediction of future outbreaks and the potential re-emergence of RVFV. Hence, we have performed the cloning, sequencing, and phylogenetic analysis, of nucleocapsid (N) protein gene. The sequence analysis showed high similarities with RVFV isolates reported from humans and animals. The highest similarity (99.5%) was observed with an isolate from Saudi Arabia (KU978775-Human) followed by 99.1% with four RVFV isolates (Human and Bovine) from other locations. A total of 51 nucleotides and 31 amino acid variations were observed throughout the N protein gene sequences. The phylogenetic relationship formed closed clusters with other isolates collected from Saudi Arabia. Thus, we report of the cloning, sequencing, and phylogenetic analysis of the RVFV-N protein gene from Saudi Arabia.
摘要：
裂谷热（RVF）是由RVFV在人类和牲畜中引起的蚊子传播的病毒性人畜共患病。目前,目前尚无批准的疫苗或抗病毒疗法.此外,在沙特阿拉伯,缺乏常规筛查系统来监测人和动物的RVFV，这阻碍了预防措施的设计和开发以及对未来疫情的预测和RVFV的潜在复发。因此，我们进行了克隆，测序,测序和系统发育分析，核衣壳(N)蛋白基因。序列分析显示与从人和动物报告的RVFV分离株高度相似性。来自沙特阿拉伯的分离株（KU978775-Human）观察到最高的相似性（99.5％），其次是99.1％，来自其他位置的四个RVFV分离株（人和牛）。在整个N蛋白基因序列中观察到总共51个核苷酸和31个氨基酸的变异。系统发育关系与从沙特阿拉伯收集的其他分离株形成了封闭簇。因此，我们报告克隆，测序,测序和来自沙特阿拉伯的RVFV-N蛋白基因的系统发育分析。
