UNASSIGNED: We collected 50 bulk milk samples from 50 dairy farms and 50 hand swabs from dairy milkers. The cefoxitin disk diffusion test and PCR-based assays were used to identify MRSA isolates. In addition, cefoxitin-resistant isolates were tested for susceptibility to other antibiotics using the Kirby-Bauer disk diffusion method.
UNASSIGNED: The results showed that MRSA was detected in 8 samples: 6 from bulk milk samples (12%) and 2 from hand swabs (4%). All MRSA isolates exhibited a high resistance rate to penicillin (100%), followed by tetracycline (75%), ciprofloxacin (25%), chloramphenicol (25%), erythromycin (25%), gentamycin (12.5%), and trimethoprim-sulfamethoxazole (12.5%). Moreover, 72% of the isolates showed resistance to three or more antibiotic classes and were classified as multidrug-resistant.
UNASSIGNED: This study identified methicillin-resistant Staphylococcus aureus and multidrug-resistant MRSA in bulk milk and dairy farms in northwestern Ethiopia. These findings highlight the potential risk of transmission of these antibiotic-resistant bacteria to humans and the need for improved antibiotic stewardship in the dairy sector using the One Health approach.
■我们从50个奶牛场收集了50个散装牛奶样品,从奶牛场收集了50个手签。使用头孢西丁纸片扩散测试和基于PCR的测定来鉴定MRSA分离株。此外,使用Kirby-Bauer圆盘扩散法测试了头孢西丁耐药菌株对其他抗生素的敏感性。
■结果表明,在8个样品中检测到MRSA:6个来自散装牛奶样品(12%),2个来自手签(4%)。所有MRSA分离株对青霉素均表现出较高的耐药率(100%),其次是四环素(75%),环丙沙星(25%),氯霉素(25%),红霉素(25%),庆大霉素(12.5%),甲氧苄啶-磺胺甲恶唑(12.5%)。此外,72%的分离株对三种或更多种抗生素具有抗性,并被归类为多重耐药。
■这项研究在埃塞俄比亚西北部的散装牛奶和奶牛场中确定了耐甲氧西林金黄色葡萄球菌和耐多药MRSA。这些发现强调了这些抗生素抗性细菌传播给人类的潜在风险,以及使用“一个健康”方法改善乳制品行业抗生素管理的必要性。