PDF(Pubmed)
Abstract：
UNASSIGNED: Infectious bronchitis (IB) is an economically important disease in poultry with worldwide distribution. The occurrence of IB has been reported both in commercial and backyard poultry in Ethiopia, although comprehensive information lacks available prevalence of the disease and the circulating serotypes.
UNASSIGNED: A cross-sectional study was conducted from November 2021 to June 2022 in seven commercial farms found in East Shewa, Central Ethiopia. Serological assay using indirect ELISA, virus isolation techniques in embryonated eggs, and molecular techniques such as one-step reverse transcriptase polymerase chain reaction (RT-PCR) and nested polymerase chain reaction (PCR) targeting a 466 bp S1 gene were employed.
UNASSIGNED: A total of 196 blood samples, 7 pools (35) of swab samples, and 5 pools of tracheal samples were investigated. The results of serological analysis revealed that 97.96% (192/196; 95% CI: 94.86-99.44) of the sera samples were found to be positive for antibodies against IBV. Out of the 7 pools of swab and 5 pools of tracheal tissue samples analyzed using RT-PCR 33.3% (4/12) of them gave positive results all from swab samples. The RT-PCR-positive samples were subjected to a nested PCR yielding 295bp and 154bp indicating the circulation of Mass and 793/B (4/91) strains of IBV, respectively. The 12 pools of samples inoculated into embryonated egg showed cytopathic changes such as congestion, bleeding, and deformation only after three passages.
UNASSIGNED: Two serotypes of IBV are circulating in Ethiopian chickens, and molecular identification of the Massachusetts serotype is the first report in Ethiopia. Further epidemiological investigation is needed in order to devise effective control measures.
UNASSIGNED: A cross-sectional study was conducted from November 2021 to June 2022 in seven commercial farms found in East Shewa, Central Ethiopia. Serological assay using indirect ELISA, virus isolation techniques in embryonated eggs, and molecular techniques such as one-step reverse transcriptase polymerase chain reaction (RT-PCR) and nested polymerase chain reaction (PCR) targeting a 466 bp S1 gene were employed.
UNASSIGNED: A total of 196 blood samples, 7 pools (35) of swab samples, and 5 pools of tracheal samples were investigated. The results of serological analysis revealed that 97.96% (192/196; 95% CI: 94.86-99.44) of the sera samples were found to be positive for antibodies against IBV. Out of the 7 pools of swab and 5 pools of tracheal tissue samples analyzed using RT-PCR 33.3% (4/12) of them gave positive results all from swab samples. The RT-PCR-positive samples were subjected to a nested PCR yielding 295bp and 154bp indicating the circulation of Mass and 793/B (4/91) strains of IBV, respectively. The 12 pools of samples inoculated into embryonated egg showed cytopathic changes such as congestion, bleeding, and deformation only after three passages.
UNASSIGNED: Two serotypes of IBV are circulating in Ethiopian chickens, and molecular identification of the Massachusetts serotype is the first report in Ethiopia. Further epidemiological investigation is needed in order to devise effective control measures.
摘要：
■传染性支气管炎(IB)是家禽中具有全球分布的经济上重要的疾病。据报道，在埃塞俄比亚的商业和后院家禽中都有IB的发生，尽管全面的信息缺乏该疾病的患病率和循环血清型。
■从2021年11月至2022年6月，在东Shewa发现的七个商业农场进行了一项横断面研究，埃塞俄比亚中部。使用间接ELISA的血清学测定，胚胎卵中的病毒分离技术，以及分子技术，例如针对466bpS1基因的一步逆转录酶聚合酶链反应（RT-PCR）和巢式聚合酶链反应（PCR）。
■总共196个血液样本，7池（35）的拭子样本，并调查了5个气管样本库。血清学分析结果表明，发现97.96％（192/196；95％CI：94.86-99.44）的血清样品对抗IBV抗体呈阳性。在使用RT-PCR分析的7个拭子池和5个气管组织样本池中,33.3%(4/12)的样本全部来自拭子样本给出阳性结果。对RT-PCR阳性样品进行巢式PCR，产生295bp和154bp，表明IBV的Mass和793/B（4/91）菌株的循环，分别。接种到胚胎卵中的12个样本池显示出细胞病变的变化，例如充血，出血,和变形后只有三个通道。
■两种IBV血清型在埃塞俄比亚鸡中循环，马萨诸塞州血清型的分子鉴定是埃塞俄比亚的第一份报告。需要进一步的流行病学调查，以制定有效的控制措施。
■从2021年11月至2022年6月，在东Shewa发现的七个商业农场进行了一项横断面研究，埃塞俄比亚中部。使用间接ELISA的血清学测定，胚胎卵中的病毒分离技术，以及分子技术，例如针对466bpS1基因的一步逆转录酶聚合酶链反应（RT-PCR）和巢式聚合酶链反应（PCR）。
■总共196个血液样本，7池（35）的拭子样本，并调查了5个气管样本库。血清学分析结果表明，发现97.96％（192/196；95％CI：94.86-99.44）的血清样品对抗IBV抗体呈阳性。在使用RT-PCR分析的7个拭子池和5个气管组织样本池中,33.3%(4/12)的样本全部来自拭子样本给出阳性结果。对RT-PCR阳性样品进行巢式PCR，产生295bp和154bp，表明IBV的Mass和793/B（4/91）菌株的循环，分别。接种到胚胎卵中的12个样本池显示出细胞病变的变化，例如充血，出血,和变形后只有三个通道。
■两种IBV血清型在埃塞俄比亚鸡中循环，马萨诸塞州血清型的分子鉴定是埃塞俄比亚的第一份报告。需要进一步的流行病学调查，以制定有效的控制措施。
