PDF(Pubmed)
Abstract：
Dispersal of infectious biofilms increases bacterial concentrations in blood. To prevent sepsis, the strength of a dispersant should be limited to allow the immune system to remove dispersed bacteria from blood, preferably without antibiotic administration. Biofilm bacteria are held together by extracellular polymeric substances that can be degraded by dispersants. Currently, comparison of the strength of dispersants is not possible by lack of a suitable comparison parameter. Here, a biofilm dispersal parameter is proposed that accounts for differences in initial biofilm properties, dispersant concentration and exposure time by using PBS as a control and normalizing outcomes with respect to concentration and time. The parameter yielded near-identical values based on dispersant-induced reductions in biomass or biofilm colony-forming-units and appeared strain-dependent across pathogens. The parameter as proposed is largely independent of experimental methods and conditions and suitable for comparing different dispersants with respect to different causative strains in particular types of infection.
摘要：
感染性生物膜的分散增加了血液中的细菌浓度。为了预防败血症,分散剂的强度应受到限制，以允许免疫系统从血液中去除分散的细菌，优选不施用抗生素。生物膜细菌由可被分散剂降解的细胞外聚合物质保持在一起。目前,由于缺乏合适的比较参数,分散剂的强度的比较是不可能的。这里,提出了一个生物膜分散参数，该参数解释了初始生物膜特性的差异，通过使用PBS作为对照和标准化结果，分散剂浓度和暴露时间。基于分散剂诱导的生物量或生物膜菌落形成单位的减少，该参数产生了几乎相同的值，并且在病原体中表现出菌株依赖性。所提出的参数在很大程度上与实验方法和条件无关，并且适合于在特定感染类型中针对不同致病菌株比较不同的分散剂。
