Abstract：
An important regulatory role for ethylene-responsive transcription factors (ERFs) is in plant growth and development, stress response, and hormone signaling. However, AP2/ERF family genes in mango have not been systematically studied. In this study, a total of 113 AP2/ERF family genes were identified from the mango genome and phylogenetically classified into five subfamilies: AP2 (28 genes), DREB (42 genes), ERF (33 genes), RAV (6 genes), and Soloist (4 genes). Of these, the ERF family, in conjunction with Arabidopsis and rice, forms a phylogenetic tree divided into seven groups, five of which have MiERF members. Analysis of gene structure and cis-elements showed that each MiERF gene contains only one AP2 structural domain, and that MiERF genes contain a large number of cis-elements associated with hormone signaling and stress response. Collinearity tests revealed a high degree of homology between MiERFs and CsERFs. Tissue-specific and stress-responsive expression profiling revealed that MiERF genes are primarily involved in the regulation of reproductive growth and are differentially and positively expressed in response to external hormones and pathogenic bacteria. Physiological results from a gain-of-function analysis of MiERF4 transiently overexpressed in tobacco and mango showed that transient expression of MiERF4 resulted in decreased colony count and callose deposition, as well as varying degrees of response to hormonal signals such as ETH, JA, and SA. Thus, MiERF4 may be involved in the JA/ETH signaling pathway to enhance plant defense against pathogenic bacteria. This study provides a basis for further research on the function and regulation of MiERF genes and lays a foundation for the selection of disease-resistant genes in mango.
摘要：
乙烯响应转录因子(ERF)在植物生长发育中的重要调节作用,应激反应，和激素信号。然而,芒果中的AP2/ERF家族基因尚未得到系统的研究。在这项研究中,从芒果基因组中鉴定出113个AP2/ERF家族基因，并将其系统发育分为5个亚家族：AP2（28个基因），DREB（42个基因），ERF（33个基因），RAV（6个基因），和独奏家（4个基因）。其中,ERF家族,与拟南芥和水稻结合，形成一个分为七个组的系统发育树，其中五个有MiERF成员。基因结构和顺式元件分析表明，每个MiERF基因仅包含一个AP2结构域，MiERF基因含有大量与激素信号和应激反应相关的顺式元件。共线性测试显示MiERF和CsERF之间高度同源性。组织特异性和应激反应表达谱显示，MiERF基因主要参与生殖生长的调节，并且在响应外部激素和病原菌时差异和阳性表达。烟草和芒果中瞬时过表达的MiERF4的功能获得分析的生理结果表明，MiERF4的瞬时表达导致菌落数量和call体沉积减少，以及对激素信号如ETH的不同程度的反应，JA,SA。因此，MiERF4可能参与JA/ETH信号通路增强植物对病原菌的防御。本研究为进一步研究芒果MiERF基因的功能和调控提供了基础,为芒果抗病基因的筛选奠定了基础。
