Abstract：
This chapter presents the integration of magnetic tweezers with single-molecule FRET technology, a significant advancement in the study of nucleic acids and other biological systems. We detail the technical aspects, challenges, and current status of this hybrid technique, which combines the global manipulation and observation capabilities of magnetic tweezers with the local conformational detection of smFRET. This innovative approach enhances our ability to analyze and understand the molecular mechanics of biological systems. The chapter serves as our first formal documentation of this method, offering insights and methodologies developed in our laboratory over the past decade.
摘要：
本章介绍了磁镊子与单分子FRET技术的集成，核酸和其他生物系统研究的重大进展。我们详细介绍了技术方面，挑战,以及这种混合技术的现状，它将磁镊子的全局操纵和观察能力与smFRET的局部构象检测相结合。这种创新方法增强了我们分析和理解生物系统分子力学的能力。这一章是我们对这种方法的第一个正式文档，提供我们实验室在过去十年中开发的见解和方法。
