METHODS: WSM was extracted and nHA was added to the matrix to construct porous composite scaffolds. The dose-effect curve of WSM concentration and alkaline phosphatase (ALP) activity was made by culturing rat osteoblasts and examining the absorbance. Three different materials were implanted into critical size defects (CSD) in the skulls of rats, which were further divided into four groups: WSM nHA /Gel group, n-WSM nHA /Gel group, HA powder group, and control group.
RESULTS: WSM (150 μg/mL-250μg/mL) effectively improved the activity of ALP in rat osteoblasts. All rats in each group had normal healing. WSM-loaded nHA /Gel group showed better performance on newly-formed bone tissue of rat skull and back at 4th week and 8th week, respectively. At the 4th week, the network of woven bone formed in the WSM-loaded nHA/Gel scaffold material. At 8th week, the reticular trabecular bone in the WSM-loaded scaffold material became dense lamellar bone, and the defect was mature lamellar bone. In the subcutaneous implantation experiment, WSM-loaded nHA/Gel scaffold material showed a better performance of heterotopic ossification than the pure nHA/Gel scaffold material.
CONCLUSIONS: WSM promotes osteoblast differentiation and bone mineralization. The results confirm that the nHA/ Gel Porous Scaffold with Nacre Water-Soluble Matrix has a significant bone promoting effect and can be used as a choice for tissue engineering to repair bone defects.
方法:提取WSM,并将nHA添加到基质中以构建多孔复合支架。通过培养大鼠成骨细胞并检测吸光度,得出WSM浓度和碱性磷酸酶(ALP)活性的剂量-效应曲线。将三种不同的材料植入大鼠头骨的临界尺寸缺陷(CSD)中,进一步分为四组:WSMnHA/Gel组,n-WSMnHA/凝胶组,HA粉组,和对照组。
结果:WSM(150μg/mL-250μg/mL)有效提高了大鼠成骨细胞中ALP的活性。各组年夜鼠均正常愈合。WSM负载nHA/Gel组在第4周和第8周时对大鼠颅骨和背部新形成的骨组织表现更好。分别。在第4周,在WSM负载的nHA/Gel支架材料中形成的编织骨网络。第8周,WSM加载的支架材料中的网状骨小梁成为致密的板层骨,缺损是成熟的板层骨。在皮下植入实验中,负载WSM的nHA/Gel支架材料比纯nHA/Gel支架材料表现出更好的异位骨化性能。
结论:WSM促进成骨细胞分化和骨矿化。结果证实,含珍珠层水溶性基质的nHA/凝胶多孔支架具有显著的骨促进作用,可作为组织工程修复骨缺损的一种选择。