PDF(Pubmed)
Abstract：
Previously, we showed that prophylactic addition of glucose to Harsha Lake water samples could inhibit cyanobacteria growth, at least for a short period of time. The current study tested cyanobacterial control with glucose for the entire Harsha Lake bloom season. Water samples (1000 ml) were collected weekly from Harsha Lake during the algal-bloom season starting June 9 and lasting until August 24, 2022. To each of two 7-liter polypropylene containers, 500 ml of Harsha Lake water was added, and the containers were placed in a controlled environment chamber. To one container labeled \"Treated,\" 0.15 g of glucose was added, and nothing was added to the container labeled \"Control.\" After that, three 25 ml samples from each container were collected and used for 16S rRNA gene sequencing each week. Then 1000 ml of Harsha Lake water was newly collected each week, with 500 ml added to each container, along with the addition of 0.15 g glucose to the \"Treated\" container. Sequencing data were used to examine differences in the composition of bacterial communities between Treated and Control containers. Treatment with glucose altered the microbial communities by 1) reducing taxonomic diversity, 2) largely eliminating cyanobacterial taxa, and 3) increasing the relative abundance of subsets of non-cyanobacterial taxa (such as Proteobacteria and Actinobacteriota). These effects were observed across time despite weekly inputs derived directly from Lake water. The addition of glucose to a container receiving weekly additions of Lake water suppressed the cyanobacterial populations during the entire summer bloom season. The glucose appears to stimulate the diversity of certain bacterial taxa at the expense of the cyanobacteria.
摘要：
以前,我们表明，在哈沙湖水样中预防性添加葡萄糖可以抑制蓝藻的生长，至少在短时间内。当前的研究测试了整个哈沙湖开花季节的葡萄糖控制蓝藻。从6月9日开始并持续到2022年8月24日的藻华季节，每周从Harsha湖收集水样（1000ml）。两个7升聚丙烯容器中的每一个，加入500毫升的哈沙湖水，并将容器放置在受控环境室中。到一个标有“已处理”的容器，“添加0.15克葡萄糖，并且没有添加到标记为“控件”的容器中。\"之后,从每个容器中收集3份25ml样本,每周用于16SrRNA基因测序.然后每周新收集1000毫升的哈沙湖水，每个容器中加入500毫升，同时向“处理过的”容器中添加0.15g葡萄糖。测序数据用于检查处理和对照容器之间细菌群落组成的差异。用葡萄糖处理通过以下方式改变了微生物群落：1）减少分类多样性，2)在很大程度上消除了蓝藻类群，和3)增加非蓝细菌类群(如变形杆菌和放线菌群)的亚群的相对丰度。尽管每周都有直接来自湖水的输入，但这些影响还是在不同时间观察到的。在整个夏季盛开季节，向每周增加湖水的容器中添加葡萄糖会抑制蓝细菌种群。葡萄糖似乎以蓝细菌为代价刺激了某些细菌类群的多样性。
