PDF(Pubmed)
Abstract：
Considering the importance of pain and stress, we decided to investigate the intra-anterior cingulate cortex (ACC) microinjection of histamine and mepyramine alone and concurrently on acute pain induced by hot plate following restraint stress in male rats. 24-gauge, 10 mm stainless steel guide cannula was implanted over the ACC in the incised scalp of 4 groups. Restraint stress in healthy rats produced a significant increase (p < .05) in the pain threshold. The simultaneous microinjection of 4 μg/side histamine and 8 μg/side mepyramine as a histaminergic system inverse agonist in healthy nonrestraint animals did not affect the pain threshold. Although Histamine decreased the threshold of pain meaningfully, mepyramine elevated it in a significant manner (p < .05). In the restrained animals, intra-ACC microinjection of histamine produced no significant impact on the pain threshold. However, intra-ACC microinjection of mepyramine before histamine, significantly (p < .01) altered the result and enhanced the threshold of pain. The results of our study demonstrated that histaminergic neurons have an important role in the processing of pain in the ACC following restraint stress.
摘要：
考虑到疼痛和压力的重要性，我们决定研究前扣带回皮质（ACC）内单独注射组胺和甲吡拉明以及同时注射对束缚应激后热板引起的急性疼痛的作用。24号仪表,在4组切开的头皮中的ACC上植入10mm不锈钢引导套管。在健康大鼠中限制应激产生疼痛阈值的显著增加(p<.05)。在健康的无束缚动物中同时显微注射4μg/侧组胺和8μg/侧美吡拉明作为组胺能系统反向激动剂不会影响疼痛阈值。虽然组胺有意义地降低了疼痛的阈值，美吡拉明以显著的方式提高了它(p<.05)。在克制的动物中，ACC内微量注射组胺对痛阈无显著影响.然而,在组胺之前，ACC内微量注射美比拉明，显着（p<.01）改变了结果并增强了疼痛的阈值。我们的研究结果表明，组胺能神经元在约束应激后ACC的疼痛处理中具有重要作用。
