Abstract：
Lumpy skin disease virus (LSDV) is a rapidly emerging pathogen in China. Screening suitable cells for LSDV replication is vital for future research on pathogenic mechanisms and vaccine development. Previous comparative studies have identified that the rodent-derived BHK21 is a highly susceptible cell model to LSDV infection. Using western blot, indirect immune-fluorescence assay, flow cytometry, and transmission electron microscopy methods, this study is the first to identify the murine osteoblastic cell line MC3T3-E1 as a novel permissive cell model for LSDV infection. The establishment of MC3T3-E1 as a suitable infectious cell model enhances our understanding of the species range and cell types of the permissive cells and nonpermissive that support LSDV replication. It is helpful to accelerate future research on the pathogenesis, clinical application, and vaccine development of LSDV.
摘要：
结节性皮肤病病毒(LSDV)是我国迅速出现的病原。筛选适合LSDV复制的细胞对于未来致病机制和疫苗开发的研究至关重要。先前的比较研究已经确定啮齿动物衍生的BHK21是对LSDV感染高度易感的细胞模型。使用蛋白质印迹，间接免疫荧光测定，流式细胞术,和透射电子显微镜方法，本研究首次将小鼠成骨细胞系MC3T3-E1确定为LSDV感染的新型允许细胞模型。MC3T3-E1作为合适的感染性细胞模型的建立增强了我们对支持LSDV复制的允许细胞和非允许细胞的物种范围和细胞类型的理解。这有助于加快未来对发病机制的研究，临床应用,和LSDV的疫苗开发。
