Abstract：
Piperine, an alkaloid found in black pepper fruits, has the properties of promoting the absorption of other substances (e.g. curcumin), therefore it is used in solid forms of dietary supplements as an additive increasing bioavailability. The aim of the study was to analyze piperine in a solid state and then to use it in the analysis of solid dietary supplements with the addition of piperine as an absorption promoter. The 13C CP MAS NMR spectra were recorded using variable contact time and dipolar dephasing experiment. The calculation of theoretical chemical shift values for three polymorphic forms of piperine allowed us to create a database of NMR parameters enabling the identification of polymorphic forms based on the analysis of the 13C CP MAS NMR spectrum. Additionally, the analysis of cross-polarization kinetics parameters was performed. Then, the 13C CP MAS NMR technique was used to confirm the authenticity and determine the presence of curcumin in dietary supplements containing curcumin with the addition of piperine. The presence of piperine could be confirmed even when the mass content of piperine was 70 times lower than that of curcumin. This method can be used to test the quality of dietary supplements containing the addition of piperine as an absorption promoter.
摘要：
胡椒碱,一种在黑胡椒果实中发现的生物碱,具有促进吸收其他物质（例如姜黄素）的特性，因此，它被用于固体形式的膳食补充剂作为增加生物利用度的添加剂。该研究的目的是分析固态的胡椒碱，然后将其用于添加胡椒碱作为吸收促进剂的固体膳食补充剂的分析。使用可变接触时间和偶极移相实验记录13CCPMASNMR光谱。对胡椒碱的三种多晶型形式的理论化学位移值的计算使我们能够创建NMR参数数据库，从而基于13CCPMASNMR光谱的分析来鉴定多晶型形式。此外,对交叉极化动力学参数进行了分析。然后，使用13CCPMASNMR技术确认真实性,并确定在添加胡椒碱的含姜黄素的膳食补充剂中是否存在姜黄素.即使当胡椒碱的质量含量比姜黄素的质量含量低70倍时,也可以确认胡椒碱的存在。该方法可用于测试含有胡椒碱作为吸收促进剂的膳食补充剂的质量。
