Abstract：
Vascular cells form a highly complex and heterogeneous tissue. Its composition, function, shape, and arrangement vary with the developmental stage and between organs and species. Understanding the transcriptional regulation underpinning this complexity thus requires a high-resolution technique that is capable of capturing rapid events during vascular cell formation. Single-cell and single-nucleus RNA sequencing (sc/snRNA-seq) approaches provide powerful tools to extract transcriptional information from these lowly abundant and dynamically changing cell types, which allows the reconstruction of developmental trajectories. Here, we summarize and reflect on recent studies using single-cell transcriptomics to study vascular cell types and discuss current and future implementations of sc/snRNA-seq approaches in the field of vascular development.
摘要：
血管细胞形成高度复杂和异质的组织。其组成,函数,形状,和排列随发育阶段以及器官和物种之间的变化而变化。因此，理解支持这种复杂性的转录调节需要一种能够捕获血管细胞形成过程中快速事件的高分辨率技术。单细胞和单核RNA测序（sc/snRNA-seq）方法提供了强大的工具，可以从这些低丰度和动态变化的细胞类型中提取转录信息。允许重建发展轨迹。这里,我们总结并反思了使用单细胞转录组学研究血管细胞类型的最新研究，并讨论了sc/snRNA-seq方法在血管发育领域的当前和未来实施。
