Abstract：
Adipose tissue provides a valuable cell source for tissue engineering, regenerative medicine, and adipose tissue biology studies. The most widely used adipose-derived stromal/stem cells (ASCs) isolation protocol involves enzymatic digestion with collagenase. However, the yield of the method often proves to be poor if not impossible for collection of sufficient stromal vascular fraction (SVF) for expansion when the sample size is small, for instance when only newborn mice are available for cell culture. Here, we describe an efficient protocol for the isolation and expansion of ASCs using explant culture as an alternative. Briefly, adipose tissue was minced after removing excess liquid. Then, the minced tissue was placed in culture dishes or flasks. The cells will migrate out of tissue and adhere to the culture surface after one or more days.
摘要：
脂肪组织为组织工程提供了有价值的细胞来源,再生医学,和脂肪组织生物学研究。最广泛使用的脂肪来源的基质/干细胞(ASC)分离方案涉及用胶原酶的酶消化。然而,该方法的产量通常被证明是差的，如果不是不可能收集足够的基质血管部分（SVF）进行扩张时，样本量小，例如，只有新生小鼠可用于细胞培养。这里,我们描述了使用外植体培养作为替代方法分离和扩增ASC的有效方案.简而言之，去除多余液体后切碎脂肪组织。然后，将切碎的组织置于培养皿或培养瓶中。一天或多天后,细胞将迁移出组织并粘附到培养物表面。
