Abstract：
Murine models of obesity or reduced adiposity are a valuable resource for understanding the role of adipocyte dysfunction in metabolic disorders. Adipose tissue stromal vascular cells or primary adipocytes derived from murine adipose tissue and grown in culture are essential tools for studying the mechanisms underlying adipocyte development and function. Herein, we describe methods for the isolation, expansion, and long-term storage of murine adipose-derived stromal/stem cells, along with protocols for inducing adipogenesis to white or beige adipocytes in this cell population and osteogenic differentiation. Isolation of the adipose stromal vascular fraction cells for flow cytometric analysis is also described.
摘要：
肥胖或肥胖减少的鼠模型是理解脂肪细胞功能障碍在代谢紊乱中的作用的宝贵资源。脂肪组织基质血管细胞或源自鼠脂肪组织并在培养物中生长的原代脂肪细胞是研究脂肪细胞发育和功能的潜在机制的重要工具。在这里，我们描述了隔离的方法，扩展,和小鼠脂肪来源的基质/干细胞的长期储存，以及诱导该细胞群中白色或米色脂肪细胞的脂肪生成和成骨分化的方案。还描述了用于流式细胞术分析的脂肪基质血管部分细胞的分离。
