PDF(Pubmed)
Abstract：
Antibiotics in animal farms play a significant role in the proliferation and spread of antibiotic-resistant genes (ARGs) and antibiotic-resistant bacteria (ARB). The dissemination of antibiotic resistance from animal facilities to the nearby environment has become an emerging concern. The present study was focused on the isolation and molecular identification of Escherichia coli (E. coli) isolates from broiler chicken meat and further access their antibiotic-resistant profile against different antibiotics. Broiler chicken meat samples were collected from 44 retail poultry slaughter shops in Prayagraj district, Uttar Pradesh, India. Standard bacteriological protocols were followed to first isolate the E. coli, and molecular characterization was performed with genus-specific PCR. Phenotypic and genotypic antibiotic-resistant profiles of all confirmed 154 E. coli isolates were screened against 09 antibiotics using the disc diffusion and PCR-based method for selected resistance genes. In antibiotic sensitivity testing, the isolates have shown maximum resistance potential against tetracycline (78%), ciprofloxacin (57.8%), trimethoprim (54.00%) and erythromycin (49.35%). E. coli bacterial isolates have shown relative resistant to amoxicillin-clavulanic acid (43.00%) and against ampicillin (44.15%). Notably, 64.28% E. coli bacteria were found to be multidrug resistant. The results of PCR assays exposed that tetA and blaTEM genes were the most abundant genes harboured by 83 (84.0%) and 82 (82.0%) out of all 99 targeted E. coli isolates, followed by 48.0% for AmpC (CITM) gene and cmlA (23.00%) for chloramphenicol resistance. It is notable that most of the isolates collected from chicken meat samples were multidrug resistant (> 3 antibiotics), with more than 80% of them carrying tetracycline (tetA) and beta-lactam gene (blaTEM). This study highlights the high risk associated with poultry products due to MDR-E. coli and promote the limited use of antibiotics in poultry farms.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s13205-024-03950-7.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s13205-024-03950-7.
摘要：
动物养殖场中的抗生素在抗生素抗性基因（ARGs）和抗生素抗性细菌（ARB）的增殖和传播中起着重要作用。抗生素抗性从动物设施传播到附近环境已成为一个新的问题。本研究的重点是大肠杆菌(E.大肠杆菌）从肉鸡鸡肉中分离出，并进一步获得其对不同抗生素的抗生素抗性谱。从Prayagraj区的44家零售家禽屠宰场收集肉鸡肉样本，北方邦,印度。遵循标准细菌学方案，首先分离大肠杆菌，用属特异性PCR进行分子鉴定。针对所选择的抗性基因,使用基于圆盘扩散和PCR的方法针对09种抗生素筛选所有确认的154个大肠杆菌分离株的表型和基因型抗生素抗性谱。在抗生素敏感性测试中，分离株对四环素显示出最大的耐药潜力(78%)，环丙沙星（57.8%），甲氧苄啶(54.00%)和红霉素(49.35%)。大肠杆菌细菌分离株对阿莫西林-克拉维酸(43.00%)和对氨苄西林(44.15%)显示出相对抗性。值得注意的是,64.28%的大肠杆菌被发现具有多重耐药性。PCR检测结果表明，tetA和blaTEM基因是所有99个目标大肠杆菌分离株中83个(84.0%)和82个(82.0%)所携带的最丰富的基因，其次是48.0％的AmpC（CITM）基因和cmlA（23.00％）的氯霉素抗性。值得注意的是，从鸡肉样品中收集的大多数分离株具有多重耐药性（>3种抗生素）。其中80％以上携带四环素（tetA）和β-内酰胺基因（blaTEM）。这项研究强调了由于MDR-E导致的与家禽产品相关的高风险。并促进家禽养殖场限制使用抗生素。
■在线版本包含补充材料，可在10.1007/s13205-024-03950-7获得。
■在线版本包含补充材料，可在10.1007/s13205-024-03950-7获得。
