PDF(Pubmed)
Abstract：
Both viral infection and vaccination affect the antibody repertoire of a person. Here, we demonstrate that the analysis of serum antibodies generates information not only on the virus type that caused the infection but also on the specific virus variant. We developed a rapid multiplex assay providing a fingerprint of serum antibodies against five different SARS-CoV-2 variants based on a microarray of virus antigens immobilized on the surface of a label-free reflectometric biosensor. We analyzed serum from the plasma of convalescent subjects and vaccinated volunteers and extracted individual antibody profiles of both total immunoglobulin Ig and IgA fractions. We found that Ig level profiles were strongly correlated with the specific variant of infection or vaccination and that vaccinated subjects displayed a larger quantity of total Ig and a lower fraction of IgA relative to the population of convalescent unvaccinated subjects.
摘要：
病毒感染和疫苗接种都会影响人的抗体库。这里,我们证明，对血清抗体的分析不仅可以产生有关导致感染的病毒类型的信息，还可以产生有关特定病毒变体的信息。我们开发了一种快速的多重测定法，该测定法基于固定在无标记反射生物传感器表面上的病毒抗原微阵列，提供了针对五种不同SARS-CoV-2变体的血清抗体指纹。我们分析了恢复期受试者和接种疫苗的志愿者的血浆中的血清，并提取了总免疫球蛋白Ig和IgA部分的个体抗体谱。我们发现Ig水平谱与感染或疫苗接种的特定变体密切相关,并且相对于恢复期未接种疫苗的受试者群体,接种疫苗的受试者显示出更大量的总Ig和更低比例的IgA。
