PDF(Pubmed)
Abstract：
To improve the genomic evaluation of milk-related traits in Holstein-Friesian (HF) cattle it is essential to identify the associated candidate genes. Novel SNP-based analyses, such as the genetic mapping of inherited diseases, GWAS, and genomic selection, have led to a new era of research. The aim of this study was to analyze the association of each individual SNP in Serbian HF cattle with milk production traits and inbreeding levels. The SNP 60 K chip Axiom Bovine BovMDv3 was deployed for the genotyping of 334 HF cows. The obtained genomic results, together with the collected phenotypic data, were used for a GWAS. Moreover, the identification of ROH segments was performed and served for inbreeding coefficient evaluation and ROH island detection. Using a GWAS, a polymorphism, rs110619097 (located in the intron of the CTNNA3 gene), was detected to be significantly (p < 0.01) associated with the milk protein concentration in the first lactation (adjusted to 305 days). The average genomic inbreeding value (FROH) was 0.079. ROH islands were discovered in proximity to genes associated with milk production traits and genomic regions under selection pressure for other economically important traits of dairy cattle. The findings of this pilot study provide useful information for a better understanding of the genetic architecture of milk production traits in Serbian HF dairy cows and can be used to improve lactation performances in Serbian HF cattle breeding programs.
摘要：
为了改善荷斯坦-弗里斯（HF）牛的牛奶相关性状的基因组评估，必须鉴定相关的候选基因。新的基于SNP的分析，比如遗传疾病的遗传图谱，GWAS,和基因组选择，导致了一个新的研究时代。这项研究的目的是分析塞尔维亚HF牛中每个SNP与产奶性状和近交水平的关联。SNP60K芯片Axiom牛BovMDv3用于334头HF母牛的基因分型。获得的基因组结果，连同收集的表型数据，用于GWAS。此外，进行了ROH片段的鉴定，并用于近交系数评估和ROH岛检测。使用GWAS，多态性，rs110619097（位于CTNNA3基因的内含子中），检测到与第一次泌乳(调整为305天)的乳蛋白浓度显著相关(p<0.01)。平均基因组近交值(FROH)为0.079。在奶牛的其他经济重要性状的选择压力下，在与产奶性状和基因组区域相关的基因附近发现了ROH岛。这项试点研究的结果为更好地了解塞尔维亚HF奶牛的产奶性状的遗传结构提供了有用的信息，并可用于改善塞尔维亚HF牛育种计划的泌乳性能。
