METHODS: Using an 8-gauge Jamshidi needle, we obtained samples from three quadrants (Q I: right margin; Q II: central; Q III: left margin) in the frontal and transverse plane and prepared these samples with a moist cloth in a 1.5 mL Eppendorf reaction vessel. The investigations were performed on a micro-CT device (SKYSCAN 1172, RJL Micro & Analytic Company, Karlsdorf-Neuthard, Germany). All collected data were analyzed using the statistical software package SPSS (version 24.0, IBM Corp., Armonk, NY, USA). Student\'s t test, the Wilcoxon-Mann-Whitney test, the Chi-squared test, and univariate analysis were used for between-group comparisons. The selection of the test depended on the number of investigated groups and the result of the Shapiro-Wilk test of normal distribution. In the case of statistically significant results, a post hoc LSD test was performed.
RESULTS: In total, we obtained 360 bone samples from 20 body donors. The craniocaudal puncture yielded data of similar magnitudes for all investigated parameters in all three quadrants, with the highest values observed in the CS. Comparisons of the ventrodorsal and craniocaudal microstructure revealed a significantly lower trabecular density and a significantly higher degree of anisotropy in the craniocaudal direction.
CONCLUSIONS: The results presented different distributions and behaviors of trabecular density, with lower density in the mid-vertebral region over the entire breadth of the vertebrae. Reduced trabecular density caused a higher degree of anisotropy and was, therefore, associated with a lower capacity to sustain biomechanical loads. Fractures in fish vertebrae were easily explained by this phenomenon. The different changes in these structures could be responsible, in part, for the changes in the TBS determined using dual-energy X-ray absorptiometry. These results confirm the clinical relevance of the TBS.
方法:使用8号Jamshidi针头,我们从正面和横向平面的三个象限(QI:右侧边缘;QII:中央;QIII:左侧边缘)获得了样品,并用湿布在1.5mLEppendorf反应容器中制备了这些样品。调查是在微型CT设备上进行的(SKYSCAN1172,RJLMicro&AnalyticCompany,Karlsdorf-Neuthard,德国)。所有收集的数据均使用统计软件包SPSS(24.0版,IBMCorp.,Armonk,NY,美国)。学生t测试,Wilcoxon-Mann-Whitney检验,卡方检验,和单变量分析用于组间比较。检验的选择取决于被调查组的数量和正态分布的Shapiro-Wilk检验的结果。在具有统计学意义的结果的情况下,进行了事后LSD测试。
结果:总计,我们从20个身体捐献者中获得了360个骨骼样本。对于所有三个象限中的所有研究参数,颅尾穿刺产生了相似幅度的数据,在CS中观察到的最高值。腹背和颅尾微结构的比较显示,骨小梁密度明显降低,颅尾方向的各向异性程度明显更高。
结论:结果显示小梁密度的分布和行为不同,在椎骨的整个宽度上,椎骨中部区域的密度较低。小梁密度的降低导致了更高的各向异性,因此,与较低的维持生物力学负荷的能力有关。这种现象很容易解释鱼椎骨的骨折。这些结构的不同变化可能是原因,在某种程度上,使用双能X射线吸收法确定的TBS的变化。这些结果证实了TBS的临床相关性。