PDF(Pubmed)
Abstract：
A major incident occurred at the Fukushima Daiichi Nuclear Power Station following the tsunami triggered by the Tohoku-Pacific Ocean Earthquake in March 2011, whereby seawater entered the torus room in the basement of the reactor building. Here, we identify and analyze the bacterial communities in the torus room water and several environmental samples. Samples of the torus room water (1 × 109 Bq137Cs/L) were collected by the Tokyo Electric Power Company Holdings from two sampling points between 30 cm and 1 m from the bottom of the room (TW1) and the bottom layer (TW2). A structural analysis of the bacterial communities based on 16S rRNA amplicon sequencing revealed that the predominant bacterial genera in TW1 and TW2 were similar. TW1 primarily contained the genus Limnobacter, a thiosulfate-oxidizing bacterium. γ-Irradiation tests on Limnobacter thiooxidans, the most closely related phylogenetically found in TW1, indicated that its radiation resistance was similar to ordinary bacteria. TW2 predominantly contained the genus Brevirhabdus, a manganese-oxidizing bacterium. Although bacterial diversity in the torus room water was lower than seawater near Fukushima, ~70% of identified genera were associated with metal corrosion. Latent environment allocation-an analytical technique that estimates habitat distributions and co-detection analyses-revealed that the microbial communities in the torus room water originated from a distinct blend of natural marine microbial and artificial bacterial communities typical of biofilms, sludge, and wastewater. Understanding the specific bacteria linked to metal corrosion in damaged plants is important for advancing decommissioning efforts.
OBJECTIVE: In the context of nuclear power station decommissioning, the proliferation of microorganisms within the reactor and piping systems constitutes a formidable challenge. Therefore, the identification of microbial communities in such environments is of paramount importance. In the aftermath of the Fukushima Daiichi Nuclear Power Station accident, microbial community analysis was conducted on environmental samples collected mainly outside the site. However, analyses using samples from on-site areas, including adjacent soil and seawater, were not performed. This study represents the first comprehensive analysis of microbial communities, utilizing meta 16S amplicon sequencing, with a focus on environmental samples collected from the radioactive element-containing water in the torus room, including the surrounding environments. Some of the identified microbial genera are shared with those previously identified in spent nuclear fuel pools in countries such as France and Brazil. Moreover, our discussion in this paper elucidates the correlation of many of these bacteria with metal corrosion.
OBJECTIVE: In the context of nuclear power station decommissioning, the proliferation of microorganisms within the reactor and piping systems constitutes a formidable challenge. Therefore, the identification of microbial communities in such environments is of paramount importance. In the aftermath of the Fukushima Daiichi Nuclear Power Station accident, microbial community analysis was conducted on environmental samples collected mainly outside the site. However, analyses using samples from on-site areas, including adjacent soil and seawater, were not performed. This study represents the first comprehensive analysis of microbial communities, utilizing meta 16S amplicon sequencing, with a focus on environmental samples collected from the radioactive element-containing water in the torus room, including the surrounding environments. Some of the identified microbial genera are shared with those previously identified in spent nuclear fuel pools in countries such as France and Brazil. Moreover, our discussion in this paper elucidates the correlation of many of these bacteria with metal corrosion.
摘要：
2011年3月东北太平洋地震引发海啸后，福岛第一核电站发生了一起重大事件，海水进入反应堆大楼地下室的环面室。这里,我们鉴定和分析了圆环室内水和几个环境样品中的细菌群落。东京电力公司控股公司从距离房间底部（TW1）和底层（TW2）30cm至1m之间的两个采样点收集了环形房间水（1×109Bq137Cs/L）。基于16SrRNA扩增子测序的细菌群落结构分析表明，TW1和TW2中的主要细菌属相似。TW1主要包含Limnobacter属，硫代硫酸盐氧化细菌。氧化硫脂林杆菌的γ辐照试验，在TW1中发现的最密切相关的系统发育学表明，其抗辐射能力与普通细菌相似。TW2主要包含Brevirhabdus属，锰氧化细菌.尽管圆环室水中的细菌多样性低于福岛附近的海水，约70%的鉴定属与金属腐蚀有关。潜在环境分配-一种估计栖息地分布和共同检测分析的分析技术-揭示了环面室内水中的微生物群落起源于天然海洋微生物和典型生物膜的人工细菌群落的独特混合物，污泥,和废水。了解与受损植物中金属腐蚀有关的特定细菌对于推进退役工作非常重要。
目的：在核电站退役的背景下，微生物在反应器和管道系统内的增殖构成了巨大的挑战。因此,在这样的环境中，微生物群落的识别是至关重要的。在福岛第一核电站事故之后，微生物群落分析是对主要在场地外收集的环境样品进行的。然而,使用现场区域的样本进行分析，包括邻近的土壤和海水，没有执行。这项研究代表了对微生物群落的首次全面分析，利用meta16S扩增子测序，重点关注从圆环室内含放射性元素的水中收集的环境样本，包括周围环境。一些已确定的微生物属与以前在法国和巴西等国家的乏核燃料池中确定的微生物属共享。此外，我们在本文中的讨论阐明了许多这些细菌与金属腐蚀的相关性。
目的：在核电站退役的背景下，微生物在反应器和管道系统内的增殖构成了巨大的挑战。因此,在这样的环境中，微生物群落的识别是至关重要的。在福岛第一核电站事故之后，微生物群落分析是对主要在场地外收集的环境样品进行的。然而,使用现场区域的样本进行分析，包括邻近的土壤和海水，没有执行。这项研究代表了对微生物群落的首次全面分析，利用meta16S扩增子测序，重点关注从圆环室内含放射性元素的水中收集的环境样本，包括周围环境。一些已确定的微生物属与以前在法国和巴西等国家的乏核燃料池中确定的微生物属共享。此外，我们在本文中的讨论阐明了许多这些细菌与金属腐蚀的相关性。
