PDF(Pubmed)
Abstract：
As a phoretic parasite and virus vector, the mite Varroa destructor and the associated Deformed wing virus (DWV) form a lethal combination to the honey bee, Apis mellifera. Routine acaricide treatment has been reported to reduce the diversity of mites and select for tolerance against these treatments. Further, different DWV strains face selective pressures when transmitted via mites. In this study, the haplotypes of Varroa mites and associated DWV variants were quantified using long reads. A single haplotype dominated the mite mitochondrial gene cytochrome oxidase subunit I, reflecting an ancient bottleneck. However, highly polymorphic genes were present across the mite genome, suggesting the diversity of mites could be actively maintained at a regional level. DWV detected in both mites and honey bees show a dominant variant with only a few low-frequency alternate haplotypes. The relative abundances of DWV haplotypes isolated from honey bees and mites were highly consistent, suggesting that some variants are favored by ongoing selection.
摘要：
作为一种电泳寄生虫和病毒载体，螨虫Varroa破坏因子和相关的变形机翼病毒（DWV）形成了对蜜蜂的致命组合，蜜蜂。据报道，常规杀螨剂治疗可减少螨虫的多样性，并选择对这些治疗的耐受性。Further,当通过螨虫传播时，不同的DWV应变面临选择性压力。在这项研究中,使用长读段对瓦螨和相关DWV变异体的单倍型进行了定量.单个单倍型主导螨线粒体基因细胞色素氧化酶亚基I，反映了一个古老的瓶颈。然而,高度多态性的基因存在于整个螨基因组中，表明可以在区域一级积极维持螨虫的多样性。在螨虫和蜜蜂中检测到的DWV均显示出显性变异，只有少数低频备用单倍型。从蜜蜂和螨虫中分离出的DWV单倍型的相对丰度高度一致，这表明一些变体受到持续选择的青睐。
