PDF(Pubmed)
Abstract：
The aim of this study was to evaluate the cholesterol lowering ability of Lactic Acid Bacteria (LAB) isolated from human breast milk under in vitro and in vivo conditions. Six LAB isolates namely Lacticaseibacillus casei 1A, Lactobacillus gasseri 5A, Enterococcus faecium 2C, Limosilactobacillus fermentum 3D, Pediococcus acidilactici 1C, and Lactiplantibacillus plantarum 7A, were examined for their bile resistance, bile salt hydrolase activity, cholesterol assimilation and viability in cholesterol rich; DeMan Rogosa and Sharpe broth, simulated gastric, small and upper intestinal conditions. During in vivo experiments, two putative LAB isolates were orally gavage to BALB/c mice, fed with normal basal and cholesterol rich (HCD) diets, daily for a period of 4 weeks. Blood serum analysis including total serum cholesterol, triglycerides, high-density and low-density lipoprotein (LDL) cholesterol levels and total fecal LAB counts of the animals were determined. The isolates in study showed bile resistance and bile salt hydrolysis activity, while significant differences (P < 0.05) were seen in their cholesterol assimilation ability. L. gasseri 5A (195.67%) and L. plantarum 7A (193.78%) displayed highest cholesterol removal percentages, respectively. Animals in HCD, fed with L. gasseri 5A and L. plantarum 7A showed decreased levels of total cholesterol and LDL, compared to the control groups. In HCD group liver weight was increased, while fecal LAB counts were decreased. No changes were observed in behavior or body weight in all experimental groups. In conclusion, L. gasseri 5A and L. plantarum 7A isolated from human breast milk demonstrates significant hypocholesterolaemic actions in vitro and in vivo and might be considered a promising candidates for preventing hypercholesterolemia in man and animals.
摘要：
这项研究的目的是评估在体外和体内条件下从人母乳中分离的乳酸菌（LAB）的降胆固醇能力。六个LAB分离株，即干酪乳杆菌1A，加氏乳杆菌5A,屎肠球菌2C，发酵肝菌,3D,酸化片球菌1C，和植物乳杆菌7A，检查了他们的胆汁抗性，胆汁盐水解酶活性，富含胆固醇的胆固醇同化和活力；DeManRogosa和Sharpe肉汤，模拟胃，小和上肠条件。在体内实验中,对BALB/c小鼠口服两种推定的LAB分离株，饲喂正常的基础和富含胆固醇（HCD）的饮食，每天，为期4周。血清分析，包括血清总胆固醇，甘油三酯，测定了动物的高密度和低密度脂蛋白（LDL）胆固醇水平以及粪便LAB总数。研究中的分离株具有胆汁抗性和胆汁盐水解活性，而他们的胆固醇同化能力存在显着差异（P<0.05）。L.gasseri5A（195.67％）和植物乳杆菌7A（193.78％）显示出最高的胆固醇去除百分比，分别。HCD中的动物，饲喂加氏乳杆菌5A和植物乳杆菌7A显示总胆固醇和LDL水平降低，与对照组相比。HCD组肝脏重量增加，而粪便LAB计数减少。在所有实验组中没有观察到行为或体重的变化。总之,从人母乳中分离的gasseri5A和植物乳杆菌7A在体内和体外均表现出显着的低胆固醇血症作用，并且可能被认为是预防人和动物高胆固醇血症的有希望的候选者。
