Abstract：
This study aims to analyze the vein of Marshall (VOM) in human autopsy hearts and its correlation with clinical data to elucidate the morphological substrates of atrial fibrillation (AF) and other cardiac diseases. Twenty-three adult autopsy hearts were studied, assessing autonomic nerves by immunohistochemistry with tyrosine hydroxylase (sympathetic nerves), choline acetyltransferase (parasympathetic nerves), growth-associated protein 43 (neural growth), and S100 (general neural marker) antibodies. Interstitial fibrosis was assessed by Masson trichrome staining. Measurements were conducted via morphometric software. The results were correlated with clinical data. Sympathetic innervation was abundant in all VOM-adjacent regions. Subjects with a history of AF, cardiovascular cause of death, and histologically verified myocardial infarction had increased sympathetic innervation and neural growth around the VOM at the mitral isthmus. Interstitial fibrosis increased with age and heart weight was associated with AF and cardiovascular cause of death. This study increases our understanding of the cardiac autonomic innervation in the VOM area in various diseases, offering implications for the development of new therapeutic approaches targeting the autonomic nervous system.
摘要：
本研究旨在分析人类尸检心脏中的马歇尔静脉（VOM）及其与临床数据的相关性，以阐明心房颤动（AF）和其他心脏病的形态学基础。研究了23个成人尸检心脏，通过酪氨酸羟化酶（交感神经）的免疫组织化学评估自主神经，胆碱乙酰转移酶（副交感神经），生长相关蛋白43（神经生长），和S100（一般神经标记）抗体。通过Masson三色染色评估间质纤维化。通过形态学软件进行测量。结果与临床数据相关。交感神经支配在所有VOM相邻区域都很丰富。有房颤病史的受试者，心血管死亡原因,经组织学证实的心肌梗死在二尖瓣峡部VOM周围的交感神经支配和神经生长增加。间质纤维化随年龄增加而增加，心脏重量与房颤和心血管死亡原因相关。这项研究增加了我们对各种疾病中VOM区域心脏自主神经支配的理解，为开发针对自主神经系统的新治疗方法提供了启示。
