PDF(Pubmed)
Abstract：
BACKGROUND: The nuclear distribution E homologue 1 (NDE1) is a crucial dynein binding partner. The NDE1 protein has the potential to disrupt the normal functioning of centrosomes, leading to a compromised ability to generate spindles and ensure precise separation of chromosomes during cell division. The potential consequences of this phenomenon include genomic instability, malignant transformation and the proliferation of neoplastic growths. However, studies examining the connection between NDE1 and cancer is still very rare.
METHODS: The expression level, prognostic impact, gene change, DNA methylation, protein interaction, mRNA m6A modification, ceRNA network, associated gene and function enrichment, and immune-related effects of NDE1 in pan-cancer were examined using a range of online analytic tools and the R software package. The CCK-8 test, transwell assay, scratch assay and colony formation assay were used to confirm the effects of NDE1 on the proliferation, invasion and metastasis of bladder cancer cells.
RESULTS: Numerous tumour types have elevated NDE1, which is linked to a bad prognosis. NDE1 is an excellent diagnostic tool for many different types of cancer. Numerous malignancies have been linked to genetic changes in NDE1. NDE1 was connected to TMB, MSI, several immunological checkpoint genes and immune cell infiltration. NDE1 is linked to a number of immunological subtypes. NDE1 could affect how well immunotherapy works to treat different types of cancer. NDE1 was mostly associated with cell cycle, chromosomal segregation, DNA replication and mitotic segregation, according to GO and KEGG analyses. NDE1 physically binds to PAFAH1B1 and DCTN1, respectively. The proliferation, invasion and metastasis of bladder cancer cells may be prevented by NDE1 knockdown. Furthermore, knockdown of NDE1 promoted the apoptosis of bladder cancer cells.
CONCLUSIONS: High expression of NDE1 is present in a variety of tumours, which is linked to a bad prognosis for cancer. Knockdown of NDE1 inhibited the proliferation, invasion and metastasis of bladder cancer cells, and promoted the apoptosis. For a number of malignancies, NDE1 may be a biomarker for immunotherapy and prognosis.
METHODS: The expression level, prognostic impact, gene change, DNA methylation, protein interaction, mRNA m6A modification, ceRNA network, associated gene and function enrichment, and immune-related effects of NDE1 in pan-cancer were examined using a range of online analytic tools and the R software package. The CCK-8 test, transwell assay, scratch assay and colony formation assay were used to confirm the effects of NDE1 on the proliferation, invasion and metastasis of bladder cancer cells.
RESULTS: Numerous tumour types have elevated NDE1, which is linked to a bad prognosis. NDE1 is an excellent diagnostic tool for many different types of cancer. Numerous malignancies have been linked to genetic changes in NDE1. NDE1 was connected to TMB, MSI, several immunological checkpoint genes and immune cell infiltration. NDE1 is linked to a number of immunological subtypes. NDE1 could affect how well immunotherapy works to treat different types of cancer. NDE1 was mostly associated with cell cycle, chromosomal segregation, DNA replication and mitotic segregation, according to GO and KEGG analyses. NDE1 physically binds to PAFAH1B1 and DCTN1, respectively. The proliferation, invasion and metastasis of bladder cancer cells may be prevented by NDE1 knockdown. Furthermore, knockdown of NDE1 promoted the apoptosis of bladder cancer cells.
CONCLUSIONS: High expression of NDE1 is present in a variety of tumours, which is linked to a bad prognosis for cancer. Knockdown of NDE1 inhibited the proliferation, invasion and metastasis of bladder cancer cells, and promoted the apoptosis. For a number of malignancies, NDE1 may be a biomarker for immunotherapy and prognosis.
摘要：
背景：核分布E同源物1（NDE1）是关键的动力蛋白结合配偶体。NDE1蛋白有可能破坏中心体的正常功能，导致在细胞分裂过程中产生纺锤体并确保染色体精确分离的能力受损。这种现象的潜在后果包括基因组不稳定，恶性转化和肿瘤生长的增殖。然而,研究NDE1与癌症之间的联系仍然非常罕见。
方法：表达水平，预后影响，基因改变,DNA甲基化，蛋白质相互作用，m6AmRNA修饰，ceRNA网络，相关基因和功能富集，使用一系列在线分析工具和R软件包检查了NDE1在泛癌症中的免疫相关作用。CCK-8测试，transwell分析，划痕试验和集落形成试验用于证实NDE1对增殖的影响，膀胱癌细胞的侵袭和转移。
结果：许多肿瘤类型的NDE1升高，这与不良预后有关。NDE1是许多不同类型癌症的优秀诊断工具。许多恶性肿瘤与NDE1的遗传变化有关。NDE1连接到TMB,MSI,几个免疫检查点基因和免疫细胞浸润。NDE1与许多免疫亚型相关。NDE1可能会影响免疫疗法治疗不同类型癌症的效果。NDE1主要与细胞周期相关，染色体分离,DNA复制和有丝分裂分离,根据GO和KEGG的分析。NDE1分别与PAFAH1B1和DCTN1物理结合。扩散,NDE1敲低可以阻止膀胱癌细胞的侵袭和转移。此外，敲除NDE1促进膀胱癌细胞凋亡。
结论：NDE1高表达存在于多种肿瘤中，这与癌症的不良预后有关。NDE1的敲减抑制增殖,膀胱癌细胞的侵袭和转移，促进细胞凋亡。对于一些恶性肿瘤，NDE1可能是免疫治疗和预后的生物标志物。
方法：表达水平，预后影响，基因改变,DNA甲基化，蛋白质相互作用，m6AmRNA修饰，ceRNA网络，相关基因和功能富集，使用一系列在线分析工具和R软件包检查了NDE1在泛癌症中的免疫相关作用。CCK-8测试，transwell分析，划痕试验和集落形成试验用于证实NDE1对增殖的影响，膀胱癌细胞的侵袭和转移。
结果：许多肿瘤类型的NDE1升高，这与不良预后有关。NDE1是许多不同类型癌症的优秀诊断工具。许多恶性肿瘤与NDE1的遗传变化有关。NDE1连接到TMB,MSI,几个免疫检查点基因和免疫细胞浸润。NDE1与许多免疫亚型相关。NDE1可能会影响免疫疗法治疗不同类型癌症的效果。NDE1主要与细胞周期相关，染色体分离,DNA复制和有丝分裂分离,根据GO和KEGG的分析。NDE1分别与PAFAH1B1和DCTN1物理结合。扩散,NDE1敲低可以阻止膀胱癌细胞的侵袭和转移。此外，敲除NDE1促进膀胱癌细胞凋亡。
结论：NDE1高表达存在于多种肿瘤中，这与癌症的不良预后有关。NDE1的敲减抑制增殖,膀胱癌细胞的侵袭和转移，促进细胞凋亡。对于一些恶性肿瘤，NDE1可能是免疫治疗和预后的生物标志物。
