PDF(Pubmed)
Abstract：
UNASSIGNED: Panax ginseng Meyer polysaccharides exhibit various biological functions, like antagonizing galectin-3-mediated cell adhesion and migration. Galectin-8 (Gal-8), with its linker-joined N- and C-terminal carbohydrate recognition domains (CRDs), is also crucial to these biological processes, and thus plays a role in various pathological disorders. Yet the effect of ginseng-derived polysaccharides in modulating Gal-8 function has remained unclear.
UNASSIGNED: P. ginseng-derived pectin was chromatographically isolated and enzymatically digested to obtain a series of polysaccharides. Biolayer Interferometry (BLI) quantified their binding affinity to Gal-8, and their inhibitory effects on Gal-8 was assessed by hemagglutination, cell migration and T-cell apoptosis.
UNASSIGNED: Our ginseng-derived pectin polysaccharides consist mostly of rhamnogalacturonan-I (RG-I) and homogalacturonan (HG). BLI shows that Gal-8 binding rests primarily in RG-I and its β-1,4-galactan side chains, with sub-micromolar KD values. Both N- and C-terminal Gal-8 CRDs bind RG-I, with binding correlated with Gal-8-mediated function.
UNASSIGNED: P. ginseng RG-I pectin β-1,4-galactan side chains are crucial to binding Gal-8 and antagonizing its function. This study enhances our understanding of galectin-sugar interactions, information that may be used in the development of pharmaceutical agents targeting Gal-8.
UNASSIGNED: P. ginseng-derived pectin was chromatographically isolated and enzymatically digested to obtain a series of polysaccharides. Biolayer Interferometry (BLI) quantified their binding affinity to Gal-8, and their inhibitory effects on Gal-8 was assessed by hemagglutination, cell migration and T-cell apoptosis.
UNASSIGNED: Our ginseng-derived pectin polysaccharides consist mostly of rhamnogalacturonan-I (RG-I) and homogalacturonan (HG). BLI shows that Gal-8 binding rests primarily in RG-I and its β-1,4-galactan side chains, with sub-micromolar KD values. Both N- and C-terminal Gal-8 CRDs bind RG-I, with binding correlated with Gal-8-mediated function.
UNASSIGNED: P. ginseng RG-I pectin β-1,4-galactan side chains are crucial to binding Gal-8 and antagonizing its function. This study enhances our understanding of galectin-sugar interactions, information that may be used in the development of pharmaceutical agents targeting Gal-8.
摘要：
■人参Meyer多糖表现出多种生物学功能，如拮抗半乳糖凝集素-3介导的细胞粘附和迁移。半乳糖凝集素-8（Gal-8），具有接头连接的N端和C端碳水化合物识别结构域（CRD），对这些生物过程也至关重要，因此在各种病理障碍中起作用。然而，人参衍生的多糖在调节Gal-8功能中的作用仍不清楚。
■P.将人参来源的果胶进行色谱分离并酶消化以获得一系列多糖。生物层干涉法（BLI）量化了它们对Gal-8的结合亲和力，并通过血凝评估了它们对Gal-8的抑制作用，细胞迁移和T细胞凋亡。
■我们的人参衍生的果胶多糖主要由鼠李糖半乳糖醛酸-I（RG-I）和高半乳糖醛酸（HG）组成。BLI显示Gal-8结合主要位于RG-I及其β-1,4-半乳聚糖侧链,具有亚微摩尔KD值。N端和C端Gal-8CRD都结合RG-I，结合与Gal-8介导的功能相关。
■P.人参RG-I果胶β-1,4-半乳聚糖侧链对于结合Gal-8和拮抗其功能至关重要。这项研究增强了我们对半乳糖凝集素-糖相互作用的理解，可用于开发靶向Gal-8的药物的信息。
■P.将人参来源的果胶进行色谱分离并酶消化以获得一系列多糖。生物层干涉法（BLI）量化了它们对Gal-8的结合亲和力，并通过血凝评估了它们对Gal-8的抑制作用，细胞迁移和T细胞凋亡。
■我们的人参衍生的果胶多糖主要由鼠李糖半乳糖醛酸-I（RG-I）和高半乳糖醛酸（HG）组成。BLI显示Gal-8结合主要位于RG-I及其β-1,4-半乳聚糖侧链,具有亚微摩尔KD值。N端和C端Gal-8CRD都结合RG-I，结合与Gal-8介导的功能相关。
■P.人参RG-I果胶β-1,4-半乳聚糖侧链对于结合Gal-8和拮抗其功能至关重要。这项研究增强了我们对半乳糖凝集素-糖相互作用的理解，可用于开发靶向Gal-8的药物的信息。
