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Abstract：
Background Dental caries is the most common bacterial disease of calcified tissues of teeth. Cariogenic biofilms formed on the tooth surface secrete organic acids and thus result in demineralization. Delving into the depth of biofilms is crucial to understand the pathogenic mechanisms and design improved therapeutic approaches. The aim of the study is to analyze the spatial and biochemical characteristics of cariogenic biofilms. Materials and methods Pulp tissue samples sourced from freshly extracted third molars were incubated with oral cariogenic bacteria namely Streptococcus mutans, Staphylococcus aureus, Escherichia coli, Entamoeba faecalis, and Candida albicans to form the biofilm. Spatial assessment of biofilms was done under FESEM (field emission scanning electron microscope, JSM-IT800, JEOL, Tokyo, Japan). FTIR (Fourier transform infrared spectroscopy, Alpha II, Bruker, Germany) spectra were assessed for chemical molecular interactions in 24- and 48-hour time periods. Results Morphological assessment with FESEM revealed rapid growth and aggregation within a short time period. FTIR spectra to analyze chemical constituents of biofilm presented with varied peaks of water, amide A, amide I, water, lipids, and phospholipids. Conclusion Further validation with more advanced imaging for an extended time period is vital to derive better conclusive evidence.
摘要：
背景龋齿是牙齿钙化组织最常见的细菌性疾病。在牙齿表面上形成的致龋生物膜分泌有机酸并因此导致脱矿质。深入研究生物膜的深度对于了解致病机制和设计改进的治疗方法至关重要。研究的目的是分析致龋生物膜的空间和生化特征。材料和方法将来自新鲜提取的第三磨牙的纸浆组织样品与口腔致龋细菌即变形链球菌一起孵育，金黄色葡萄球菌,大肠杆菌,粪内变形虫，和白色念珠菌形成生物膜。生物膜的空间评估是在FESEM(场发射扫描电子显微镜，JSM-IT800,JEOL,东京,日本）。FTIR(傅里叶变换红外光谱,阿尔法II,布鲁克,德国）的光谱在24小时和48小时的时间段内评估了化学分子的相互作用。结果用FESEM进行的形态学评估显示在短时间内快速生长和聚集。用FTIR光谱分析生物膜的化学成分，并呈现不同的水峰，酰胺A,酰胺I,水,脂质，和磷脂。结论在延长的时间内进行更先进的成像进一步验证对于获得更好的结论性证据至关重要。
