PDF(Pubmed)
Abstract：
UNASSIGNED: Spinal cord injury (SCI) is a profoundly disabling and devastating neurological condition, significantly impacting patients\' quality of life. It imposes unbearable psychological and economic pressure on both patients and their families, as well as placing a heavy burden on society.
UNASSIGNED: In this study, we integrated datasets GSE5296 and GSE47681 as training groups, analyzed gene variances between sham group and SCI group mice, and conducted Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis based on the differentially expressed genes. Subsequently, we performed Weighted Gene Correlation Network Analysis (WGCNA) and Lasso regression analyses.
UNASSIGNED: We identified four characteristic disease genes: Icam1, Ch25h, Plaur and Tm4sf1. We examined the relationship between SCI and immune cells, and validated the expression of the identified disease-related genes in SCI rats using PCR and immunohistochemistry experiments.
UNASSIGNED: In conclusion, we have identified and verified four genes related to SCI: Icam1, Ch25h, Plaur and Tm4sf1, which could offer insights for SCI treatment.
UNASSIGNED: In this study, we integrated datasets GSE5296 and GSE47681 as training groups, analyzed gene variances between sham group and SCI group mice, and conducted Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis based on the differentially expressed genes. Subsequently, we performed Weighted Gene Correlation Network Analysis (WGCNA) and Lasso regression analyses.
UNASSIGNED: We identified four characteristic disease genes: Icam1, Ch25h, Plaur and Tm4sf1. We examined the relationship between SCI and immune cells, and validated the expression of the identified disease-related genes in SCI rats using PCR and immunohistochemistry experiments.
UNASSIGNED: In conclusion, we have identified and verified four genes related to SCI: Icam1, Ch25h, Plaur and Tm4sf1, which could offer insights for SCI treatment.
摘要：
■脊髓损伤（SCI）是一种严重致残和毁灭性的神经系统疾病，显著影响患者生活质量。它给患者和家属带来难以承受的心理和经济压力,给社会带来沉重负担。
■在这项研究中，我们整合了数据集GSE5296和GSE47681作为训练组，分析假手术组和SCI组小鼠之间的基因突变，并基于差异表达基因进行了基因本体论（GO）富集分析和京都基因和基因组百科全书（KEGG）富集分析。随后，我们进行了加权基因相关网络分析(WGCNA)和Lasso回归分析.
■我们确定了四个特征性疾病基因：Icam1，Ch25h，Plaur和Tm4sf1。我们检查了SCI和免疫细胞之间的关系，并通过PCR和免疫组织化学实验验证了已鉴定的疾病相关基因在SCI大鼠中的表达。
■总而言之，我们已经确定并验证了与SCI相关的四个基因：Icam1，Ch25h，Plaur和Tm4sf1，可以为SCI治疗提供见解。
■在这项研究中，我们整合了数据集GSE5296和GSE47681作为训练组，分析假手术组和SCI组小鼠之间的基因突变，并基于差异表达基因进行了基因本体论（GO）富集分析和京都基因和基因组百科全书（KEGG）富集分析。随后，我们进行了加权基因相关网络分析(WGCNA)和Lasso回归分析.
■我们确定了四个特征性疾病基因：Icam1，Ch25h，Plaur和Tm4sf1。我们检查了SCI和免疫细胞之间的关系，并通过PCR和免疫组织化学实验验证了已鉴定的疾病相关基因在SCI大鼠中的表达。
■总而言之，我们已经确定并验证了与SCI相关的四个基因：Icam1，Ch25h，Plaur和Tm4sf1，可以为SCI治疗提供见解。
