UNASSIGNED: This research was aimed to assess the effect of collagen cross-linking agents on the integrity of radicular collagen matrix and depth of penetration of sealer.
UNASSIGNED: Mandibular premolars (n = 48) were taken. Teeth were decoronated; canals were prepared till ProTaper size F2 and were irrigated with 5 mL of 2.5% NaOCl, followed by 3 mL of 17% ethylenediaminetetraacetic acid between instrumentation and finally rinsed with saline following which teeth were divided into three groups based on the surface treatments: Group 1: 6.5% proanthocyanin (PA), Group 2: chlorhexidine (CHX), and Group 3: saline. Teeth were obturated using gutta-percha and bioceramic sealer and stored in artificial saliva. Hydroxyproline (HYP) release was assessed after 14 and 21 days using spectrophotometer. Sealer penetration was assessed using the scanning electron microscope.
UNASSIGNED: Wilcoxon signed-rank test and Kruskal-Wallis test for release of HYP and paired t-test and ANOVA for sealer penetration were performed.
UNASSIGNED: Significantly lower release of HYP was seen in proanthocyanin-treated group. Sealer penetration was better for both the proanthocyanin- and CHX-treated groups when compared to saline.
UNASSIGNED: Surface treatment with collagen cross-linkers caused a decrease in the amount of HYP released, indicating lesser degradation of collagen. Sealer penetration was better due to the removal of smear layer following the surface treatments.
■这项研究旨在评估胶原蛋白交联剂对神经根胶原蛋白基质完整性和密封剂渗透深度的影响。
■取下颌前磨牙(n=48)。对牙齿进行装饰;准备运河直到ProTaper尺寸F2,并用5mL2.5%NaOCl灌溉,然后在仪器之间加入3毫升17%的乙二胺四乙酸,最后用盐水冲洗,然后根据表面处理将牙齿分为三组:第1组:6.5%原花青素(PA),第2组:氯己定(CHX),第3组:生理盐水。使用牙胶和生物陶瓷密封剂封闭牙齿,并储存在人工唾液中。在14天和21天后使用分光光度计评估羟脯氨酸(HYP)释放。使用扫描电子显微镜评估密封剂渗透。
■进行HYP释放的Wilcoxon符号秩检验和Kruskal-Wallis检验以及封口剂渗透的配对t检验和方差分析。
■在原花青素处理组中观察到HYP的释放显著降低。与盐水相比,原花青素和CHX处理组的密封剂渗透更好。
■用胶原蛋白交联剂进行表面处理导致HYP释放量减少,表明胶原蛋白降解较少。由于在表面处理之后去除涂抹层,密封剂渗透更好。