PDF(Pubmed)
Abstract：
Lovebird (Agapornis personatus) is a monotypic species of bird of the lovebird genus in the parrot family Psittaculidae and order Psittaciformes.
The present study was designed to investigate the histology and immunohistochemistry of the pancreas in the lovebird.
Totally, three adult birds were used. The pancreas was assessed using histological and immunofluorescent staining to detect insulin, glucagon, somatostatin, pancreatic polypeptide (PP) and neuropeptide Y (NY).
The exocrine pancreas was composed of pyramidal acinar cells with zymogen granules at the apical cytoplasm. The endocrine pancreas was identified as large alpha, small beta and mixed islets of Langerhans. No intercalated duct was observed. Alpha cells with a density of 28.55% were the most numerous cell type, which were populated throughout the large islets, especially at the periphery. The beta cells with a density of 15.78% were accumulated mostly at the periphery of islets. The delta cells exhibited 17.81% intensity. Despite their lower density, the distribution of delta cells was like that of A cells throughout the islets. PP and NY cells were distinguished with densities of 14.69% and 20.63%, respectively.
Although the arrangement of acinar cells, ductal systems and endocrine islets reflects patterns observed in various avian species, the absence of intercalated duct, the presence of three types of Langerhans islets as alpha, beta and mixed islets and the high expression of NY in the islets were some unique features observed in the current study. These findings contribute to the broader understanding of avian pancreas histology.
The present study was designed to investigate the histology and immunohistochemistry of the pancreas in the lovebird.
Totally, three adult birds were used. The pancreas was assessed using histological and immunofluorescent staining to detect insulin, glucagon, somatostatin, pancreatic polypeptide (PP) and neuropeptide Y (NY).
The exocrine pancreas was composed of pyramidal acinar cells with zymogen granules at the apical cytoplasm. The endocrine pancreas was identified as large alpha, small beta and mixed islets of Langerhans. No intercalated duct was observed. Alpha cells with a density of 28.55% were the most numerous cell type, which were populated throughout the large islets, especially at the periphery. The beta cells with a density of 15.78% were accumulated mostly at the periphery of islets. The delta cells exhibited 17.81% intensity. Despite their lower density, the distribution of delta cells was like that of A cells throughout the islets. PP and NY cells were distinguished with densities of 14.69% and 20.63%, respectively.
Although the arrangement of acinar cells, ductal systems and endocrine islets reflects patterns observed in various avian species, the absence of intercalated duct, the presence of three types of Langerhans islets as alpha, beta and mixed islets and the high expression of NY in the islets were some unique features observed in the current study. These findings contribute to the broader understanding of avian pancreas histology.
摘要：
背景：Lovebird（Agapornispersonatus）是鹦鹉科Psittaculidae和Psittaciformes目中的Lovebird属鸟类的单型物种。
目的：本研究旨在研究爱情鸟胰腺的组织学和免疫组织化学。
方法：完全，使用了三只成年鸟。胰腺采用组织学和免疫荧光染色检测胰岛素，胰高血糖素,生长抑素,胰腺多肽（PP）和神经肽Y（NY）。
结果：胰腺外分泌由锥体细胞腺泡细胞和顶端细胞质的酶原颗粒组成。内分泌胰腺被鉴定为大α，小β和朗格汉斯混合胰岛。没有观察到插层导管。密度为28.55％的α细胞是最多的细胞类型，它们遍布在大小岛上，尤其是在外围。密度为15.78％的β细胞主要在胰岛周围积累。δ细胞表现出17.81%的强度。尽管密度较低，δ细胞在整个胰岛中的分布类似于A细胞。PP和NY细胞的密度分别为14.69％和20.63％，分别。
结论：尽管腺泡细胞的排列，导管系统和内分泌胰岛反映了在各种鸟类中观察到的模式，没有插层管道，存在三种类型的朗格汉斯胰岛作为阿尔法，β和混合胰岛以及NY在胰岛中的高表达是当前研究中观察到的一些独特特征。这些发现有助于对禽胰腺组织学的更广泛理解。
目的：本研究旨在研究爱情鸟胰腺的组织学和免疫组织化学。
方法：完全，使用了三只成年鸟。胰腺采用组织学和免疫荧光染色检测胰岛素，胰高血糖素,生长抑素,胰腺多肽（PP）和神经肽Y（NY）。
结果：胰腺外分泌由锥体细胞腺泡细胞和顶端细胞质的酶原颗粒组成。内分泌胰腺被鉴定为大α，小β和朗格汉斯混合胰岛。没有观察到插层导管。密度为28.55％的α细胞是最多的细胞类型，它们遍布在大小岛上，尤其是在外围。密度为15.78％的β细胞主要在胰岛周围积累。δ细胞表现出17.81%的强度。尽管密度较低，δ细胞在整个胰岛中的分布类似于A细胞。PP和NY细胞的密度分别为14.69％和20.63％，分别。
结论：尽管腺泡细胞的排列，导管系统和内分泌胰岛反映了在各种鸟类中观察到的模式，没有插层管道，存在三种类型的朗格汉斯胰岛作为阿尔法，β和混合胰岛以及NY在胰岛中的高表达是当前研究中观察到的一些独特特征。这些发现有助于对禽胰腺组织学的更广泛理解。
