PDF(Pubmed)
Abstract：
The objective of this study was to elaborate Doppler ultrasonographic scan, genetic resistance and serum profile of markers associated with endometritis susceptibility in Egyptian buffalo-cows. The enrolled animals were designed as; twenty five apparently healthy buffalo-cows considered as a control group and twenty five infected buffalo with endometritis. There were significant (p < 0.05) increased of cervical diameter, endometrium thickness, uterine horn diameter, TAMEAN, TAMAX and blood flow through middle uterine artery with significant decrease of PI and RI values in endometritis buffalo-cows. Gene expression levels were considerably higher in endometritis-affected buffaloes than in resistant ones for the genes A2M, ADAMTS20, KCNT2, MAP3K4, MAPK14, FKBP5, FCAMR, TLR2, IRAK3, CCl2, EPHA4, and iNOS. The RXFP1, NDUFS5, TGF-β, SOD3, CAT, and GPX genes were expressed at substantially lower levels in endometritis-affected buffaloes. The PCR-DNA sequence verdicts of healthy and affected buffaloes revealed differences in the SNPs in the amplified DNA bases related to endometritis for the investigated genes. However, MAP3K4 elicited a monomorphic pattern. There was a significant decrease of red blood cells (RBCs) count, Hb and packed cell volume (PCV) with neutrophilia, lymphocytosis and monocytosis in endometritis group compared with healthy ones. The serum levels of Hp, SAA, Cp, IL-6, IL-10, TNF-α, NO and MDA were significantly (P˂0.05) increased, along with reduction of CAT, GPx, SOD and TAC in buffalo-cows with endometritis compared to healthy ones. The variability of Doppler ultrasonographic scan and studied genes alongside alterations in the serum profile of investigated markers could be a reference guide for limiting buffalo endometritis through selective breeding of natural resistant animals.
摘要：
这项研究的目的是详细的多普勒超声扫描，埃及水牛中与子宫内膜炎易感性相关的标志物的遗传抗性和血清谱。所登记的动物被设计为:25头明显健康的水牛被认为是对照组,25头感染子宫内膜炎的水牛被认为是对照组。宫颈直径显著增加(p<0.05),子宫内膜厚度,子宫角直径,Tamean,水牛子宫内膜炎的TAMAX和通过子宫中动脉的血流与PI和RI值显着降低。受子宫内膜炎影响的水牛的基因表达水平明显高于A2M基因的抗性水牛，ADAMTS20,KCNT2,MAP3K4,MAPK14,FKBP5,FCAMR,TLR2、IRAK3、CCl2、EPHA4和iNOS。RXFP1，NDUFS5，TGF-β，SOD3,CAT,在受子宫内膜炎影响的水牛中，GPX基因的表达水平大大降低。健康和受影响的水牛的PCR-DNA序列判决揭示了所研究基因与子宫内膜炎相关的扩增DNA碱基中的SNP差异。然而,MAP3K4引发了单态模式。红细胞（RBC）计数显着下降，Hb和填充细胞体积（PCV）与中性粒细胞增多症，子宫内膜炎组的淋巴细胞和单核细胞增多症与健康者相比。血清Hp水平,SAA,Cp,IL-6，IL-10，TNF-α，NO和MDA显著升高(P<0.05),随着CAT的减少，GPx,子宫内膜炎水牛与健康牛的SOD和TAC比较。多普勒超声扫描和研究基因的变异性以及所研究标记物血清谱的变化，可以作为通过选择性育种自然抗性动物来限制水牛子宫内膜炎的参考指南。
