Abstract：
X-ray crystallography and cryo-electron microscopy have enabled the determination of structures of numerous viruses at high resolution and have greatly advanced the field of structural virology. These structures represent only a subset of snapshot end-state conformations, without describing all conformational transitions that virus particles undergo. Allostery plays a critical role in relaying the effects of varied perturbations both on the surface through environmental changes and protein (receptor/antibody) interactions into the genomic core of the virus. Correspondingly, allostery carries implications for communicating changes in genome packaging to the overall stability of the virus particle. Amide hydrogen/deuterium exchange mass spectrometry (HDXMS) of whole viruses is a powerful probe for uncovering virus allostery. Here we critically discuss advancements in understanding virus dynamics by HDXMS with single particle cryo-EM and computational approaches.
摘要：
X射线晶体学和低温电子显微镜已经能够以高分辨率确定许多病毒的结构，并且极大地推进了结构病毒学领域。这些结构仅代表快照最终状态构象的子集，没有描述病毒颗粒经历的所有构象转变。在通过环境变化和蛋白质（受体/抗体）相互作用将表面上的各种扰动的影响传递到病毒的基因组核心中起着关键作用。相应地,变形外科对于将基因组包装的变化传达给病毒颗粒的整体稳定性具有重要意义。全病毒的酰胺氢/氘交换质谱（HDXMS）是揭示病毒变形性的强大探针。在这里，我们批判性地讨论了通过HDXMS使用单粒子cryo-EM和计算方法来理解病毒动力学的进展。
