UNASSIGNED: The effects of the combined treatments (5 μM oxa + 50 μM pun, 5 μM oxa + 75 μM pun, 20 μM oxa + 50 μM pun, and 5 μM oxa + 75 μM pun) were compared with untreated Caco2 cells (control) or cells treated with oxa alone. Apoptosis was detected using an Annex in V FITC flow cytometry assay and poly (ADP-ribose) polymerase cleavage by western blotting. Light chain 3 was detected by western blotting as an autophagy marker.
UNASSIGNED: The combined treatments significantly increased the number of apoptotic cells in comparison to untreated cells or cells treated with oxa alone. By contrast, the combined treatments had no significant effect on autophagy.
UNASSIGNED: The combined treatment significantly promoted cell death through apoptosis while maintaining a basal level of autophagy.
■联合治疗的效果(5μMoxa+50μM双关,5μMoxa+75μM双关语,20μMoxa+50μM双关语,和5μMoxa75μMpun)与未处理的Caco2细胞(对照)或单独用oxa处理的细胞进行比较。使用V中的附件FITC流式细胞术测定法和通过蛋白质印迹的聚(ADP-核糖)聚合酶裂解来检测细胞凋亡。通过蛋白质印迹检测轻链3作为自噬标记。
与未处理的细胞或单独用oxa处理的细胞相比,组合处理显著增加了凋亡细胞的数量。相比之下,联合治疗对自噬无明显影响。
■联合治疗通过凋亡显着促进细胞死亡,同时维持自噬的基础水平。