PDF(Pubmed)
Abstract：
UNASSIGNED: The objectives of the study are to investigate the synergistic effect of oxaliplatin (oxa) and punicalagin (pun) on the death of colon cancer cells (Caco-2) by apoptosis and autophagy.
UNASSIGNED: The effects of the combined treatments (5 μM oxa + 50 μM pun, 5 μM oxa + 75 μM pun, 20 μM oxa + 50 μM pun, and 5 μM oxa + 75 μM pun) were compared with untreated Caco2 cells (control) or cells treated with oxa alone. Apoptosis was detected using an Annex in V FITC flow cytometry assay and poly (ADP-ribose) polymerase cleavage by western blotting. Light chain 3 was detected by western blotting as an autophagy marker.
UNASSIGNED: The combined treatments significantly increased the number of apoptotic cells in comparison to untreated cells or cells treated with oxa alone. By contrast, the combined treatments had no significant effect on autophagy.
UNASSIGNED: The combined treatment significantly promoted cell death through apoptosis while maintaining a basal level of autophagy.
UNASSIGNED: The effects of the combined treatments (5 μM oxa + 50 μM pun, 5 μM oxa + 75 μM pun, 20 μM oxa + 50 μM pun, and 5 μM oxa + 75 μM pun) were compared with untreated Caco2 cells (control) or cells treated with oxa alone. Apoptosis was detected using an Annex in V FITC flow cytometry assay and poly (ADP-ribose) polymerase cleavage by western blotting. Light chain 3 was detected by western blotting as an autophagy marker.
UNASSIGNED: The combined treatments significantly increased the number of apoptotic cells in comparison to untreated cells or cells treated with oxa alone. By contrast, the combined treatments had no significant effect on autophagy.
UNASSIGNED: The combined treatment significantly promoted cell death through apoptosis while maintaining a basal level of autophagy.
摘要：
■该研究的目的是研究奥沙利铂（oxa）和punicalagin（pun）通过凋亡和自噬对结肠癌细胞（Caco-2）死亡的协同作用。
■联合治疗的效果（5μMoxa+50μM双关，5μMoxa+75μM双关语，20μMoxa+50μM双关语，和5μMoxa75μMpun）与未处理的Caco2细胞（对照）或单独用oxa处理的细胞进行比较。使用V中的附件FITC流式细胞术测定法和通过蛋白质印迹的聚(ADP-核糖)聚合酶裂解来检测细胞凋亡。通过蛋白质印迹检测轻链3作为自噬标记。
与未处理的细胞或单独用oxa处理的细胞相比,组合处理显著增加了凋亡细胞的数量。相比之下,联合治疗对自噬无明显影响。
■联合治疗通过凋亡显着促进细胞死亡，同时维持自噬的基础水平。
■联合治疗的效果（5μMoxa+50μM双关，5μMoxa+75μM双关语，20μMoxa+50μM双关语，和5μMoxa75μMpun）与未处理的Caco2细胞（对照）或单独用oxa处理的细胞进行比较。使用V中的附件FITC流式细胞术测定法和通过蛋白质印迹的聚(ADP-核糖)聚合酶裂解来检测细胞凋亡。通过蛋白质印迹检测轻链3作为自噬标记。
与未处理的细胞或单独用oxa处理的细胞相比,组合处理显著增加了凋亡细胞的数量。相比之下,联合治疗对自噬无明显影响。
■联合治疗通过凋亡显着促进细胞死亡，同时维持自噬的基础水平。
