Abstract：
Epidemics caused by multiple viruses continue to emerge, which have brought a terrible impact on human society. Identification of viral infections with high sensitivity and portability is of significant importance for the screening and management of diseases caused by viruses. Herein, a microfluidic chip (MFC)-assisted upconversion luminescence biosensing platform is designed and fabricated for point-of-care virus detection. Upconversion nanoparticles with excellent stability are successfully synthesized as luminescent agents for optical signal generation in the portable virus diagnostic platform. The relevant investigation results illustrate that the MFC-assisted virus diagnostic platform possesses outstanding performance such as good integration, high sensitivity (1.12 pg mL-1), ease of use, and portability. In addition, clinical sample test result verifies its more prominent virus diagnostic properties than commercially available rapid test strips. All of these thrilling capabilities imply that the designed portable virus diagnostic platform has great potential for future virus detection applications.
摘要：
由多种病毒引起的疫情不断出现,给人类社会带来了可怕的影响。以高灵敏度和便携性鉴定病毒感染对于筛选和管理由病毒引起的疾病具有重要意义。在这里，微流控芯片（MFC）辅助的上转换发光生物传感平台已被设计和制造用于即时病毒检测。具有优异稳定性的上转换纳米颗粒被成功合成为发光剂,用于在便携式病毒诊断平台中产生光信号。相关调查结果表明,MFC辅助病毒诊断平台具有良好的集成性,高灵敏度（1.12pg/ml），易用性,和便携性。此外,临床样品测试结果验证了其比市售快速测试条更突出的病毒诊断特性。所有这些令人兴奋的功能都意味着我们设计的便携式病毒诊断平台在未来的病毒检测应用中具有巨大的潜力。本文受版权保护。保留所有权利。
