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Abstract：
The objective of the present study was to investigate the potential role of immunization against INH on follicular development, serum reproductive hormone (FSH, E2, and P4) concentrations, and reproductive performance in beef cattle. A total of 196 non-lactating female beef cattle (4-5 years old) with identical calving records (3 records) were immunized with 0.5, 1.0, 1.5, or 2.0 mg [(T1, n = 58), (T2, n = 46), (T3, n = 42) and (T4, n = 36), respectively] of the pcISI plasmid. The control (C) group (n = 14) was immunized with 1.0 mL 0.9% saline. At 21d after primary immunization, all beef cattle were boosted with half of the primary immunization dose. On day 10 after primary immunization, the beef cattle immunized with INH DNA vaccine evidently induced anti-INH antibody except for the T1 group. The T3 group had the greatest P/N value peak among all the groups. The anti-INH antibody positive rates in T2, T3 and T4 groups were significantly higher than that in C and T1 groups. RIA results indicated that serum FSH concentration in T2 group increased markedly on day 45 after booster immunization; the E2 amount in T3 group was significantly increased on day 10 after primary immunization, and the levels of E2 also improved in T2 and T3 groups after booster immunization; the P4 concentration in T2 group was significantly improved on day 21 after primary immunization. Ultrasonography results revealed that the follicles with different diameter sizes were increased, meanwhile, the diameter and growth speed of ovulatory follicle were significantly increased. Furthermore, the rates of estrous, ovulation, conception, and twinning rate were also significantly enhanced. These findings clearly illustrated that INH DNA vaccine was capable of promoting the follicle development, thereby improving the behavioral of estrous and ovulation, eventually leading to an augment in the conception rates and twinning rate of beef cattle.
摘要：
本研究的目的是探讨INH免疫对卵泡发育的潜在作用，血清生殖激素（FSH，E2和P4)浓度，和肉牛的繁殖性能。用0.5、1.0、1.5或2.0mg[（T1，n=58）免疫接种了具有相同产牛记录（3个记录）的196头非哺乳期雌性肉牛（4-5岁），（T2，n=46），（T3，n=42）和（T4，n=36），分别]的pcISI质粒。对照(C)组(n=14)用1.0mL0.9%盐水免疫。初次免疫后21d，所有肉牛都被一次免疫剂量的一半所加强。初次免疫后第10天，除T1组外，用INHDNA疫苗免疫的肉牛明显诱导了抗INH抗体。T3组P/N值峰值最大。T2、T3、T4组抗INH抗体阳性率明显高于C、T1组。RIA结果表明，T2组血清FSH浓度在加强免疫后第45天明显升高；T3组E2含量在初次免疫后第10天明显升高。强化免疫后T2和T3组的E2水平也有所提高；初次免疫后第21天，T2组的P4浓度显着提高。超声检查结果显示不同直径大小的卵泡增大,同时,排卵卵泡直径和生长速度显著增加。此外，发情率，排卵,概念,孪生率也显著提高。这些发现清楚地表明，INHDNA疫苗能够促进卵泡发育，从而改善发情和排卵的行为，最终导致肉牛的受胎率和孪生率增加。
