PDF(Pubmed)
Abstract：
UNASSIGNED: This study aims to correlate caries-causing microorganism load, lactic acid estimation, and blood groups to high caries risk in diabetic and non-diabetic individuals and low caries risk in healthy individuals.
UNASSIGNED: This study includes 30 participants divided into 3 groups: Group A, High-risk caries diabetic individuals; Group B, High-risk caries non-diabetic individuals; and Group C, Low-risk caries individuals. The medical condition, oral hygiene, and caries risk assessment (American Dental Association classification and International Caries Detection and Assessment System scoring) were documented. Each individual\'s 3 mL of saliva was analyzed for microbial load and lactic acid as follows: Part I: 2 mL for microbial quantity estimation using nutrient agar and blood agar medium, biochemical investigation, and carbohydrate fermentation tests; Part II: 0.5 mL for lactic acid estimation using spectrophotometric analysis. Among the selected individuals, blood group correlation was assessed. The χ2 test, Kruskal-Wallis test, and post hoc analysis were done using Dunn\'s test (p < 0.05).
UNASSIGNED: Group A had the highest microbial load and lactic acid concentration, followed by Groups B and C. The predominant bacteria were Lactobacilli (63.00 ± 15.49) and Streptococcus mutans (76.00 ± 13.90) in saliva. Blood Group B is prevalent in diabetic and non-diabetic high-risk caries patients but statistically insignificant.
UNASSIGNED: Diabetic individuals are more susceptible to dental caries due to high microbial loads and increased lactic acid production. These factors also lower the executing tendency of neutrophils, which accelerates microbial accumulation and increases the risk of caries in diabetic individuals.
UNASSIGNED: This study includes 30 participants divided into 3 groups: Group A, High-risk caries diabetic individuals; Group B, High-risk caries non-diabetic individuals; and Group C, Low-risk caries individuals. The medical condition, oral hygiene, and caries risk assessment (American Dental Association classification and International Caries Detection and Assessment System scoring) were documented. Each individual\'s 3 mL of saliva was analyzed for microbial load and lactic acid as follows: Part I: 2 mL for microbial quantity estimation using nutrient agar and blood agar medium, biochemical investigation, and carbohydrate fermentation tests; Part II: 0.5 mL for lactic acid estimation using spectrophotometric analysis. Among the selected individuals, blood group correlation was assessed. The χ2 test, Kruskal-Wallis test, and post hoc analysis were done using Dunn\'s test (p < 0.05).
UNASSIGNED: Group A had the highest microbial load and lactic acid concentration, followed by Groups B and C. The predominant bacteria were Lactobacilli (63.00 ± 15.49) and Streptococcus mutans (76.00 ± 13.90) in saliva. Blood Group B is prevalent in diabetic and non-diabetic high-risk caries patients but statistically insignificant.
UNASSIGNED: Diabetic individuals are more susceptible to dental caries due to high microbial loads and increased lactic acid production. These factors also lower the executing tendency of neutrophils, which accelerates microbial accumulation and increases the risk of caries in diabetic individuals.
摘要：
■这项研究旨在关联引起龋齿的微生物负荷，乳酸估算，糖尿病和非糖尿病个体患龋风险高，健康个体患龋风险低。
■这项研究包括30名参与者，分为3组：A组，高风险龋齿糖尿病个体；B组，高风险龋齿非糖尿病个体；和C组，低风险龋齿个体。医疗状况,口腔卫生,并记录龋齿风险评估（美国牙科协会分类和国际龋齿检测和评估系统评分）。每个人的3毫升唾液的微生物负荷和乳酸分析如下：第一部分：2毫升微生物数量估计使用营养琼脂和血琼脂培养基，生化调查，和碳水化合物发酵试验；第二部分：使用分光光度分析进行乳酸估算的0.5mL。在选定的个人中，评估血型相关性。χ2检验,Kruskal-Wallis测试,和事后分析使用Dunn检验(p<0.05)。
■A组的微生物负荷和乳酸浓度最高，其次是B组和C组。唾液中的优势细菌是乳杆菌（63.00±15.49）和变形链球菌（76.00±13.90）。血型B在糖尿病和非糖尿病高危龋齿患者中普遍存在，但统计学上无统计学意义。
■由于高微生物负荷和乳酸产生增加，糖尿病个体更容易患龋齿。这些因素也降低了中性粒细胞的执行趋势，这加速了微生物的积累并增加了糖尿病个体患龋齿的风险。
■这项研究包括30名参与者，分为3组：A组，高风险龋齿糖尿病个体；B组，高风险龋齿非糖尿病个体；和C组，低风险龋齿个体。医疗状况,口腔卫生,并记录龋齿风险评估（美国牙科协会分类和国际龋齿检测和评估系统评分）。每个人的3毫升唾液的微生物负荷和乳酸分析如下：第一部分：2毫升微生物数量估计使用营养琼脂和血琼脂培养基，生化调查，和碳水化合物发酵试验；第二部分：使用分光光度分析进行乳酸估算的0.5mL。在选定的个人中，评估血型相关性。χ2检验,Kruskal-Wallis测试,和事后分析使用Dunn检验(p<0.05)。
■A组的微生物负荷和乳酸浓度最高，其次是B组和C组。唾液中的优势细菌是乳杆菌（63.00±15.49）和变形链球菌（76.00±13.90）。血型B在糖尿病和非糖尿病高危龋齿患者中普遍存在，但统计学上无统计学意义。
■由于高微生物负荷和乳酸产生增加，糖尿病个体更容易患龋齿。这些因素也降低了中性粒细胞的执行趋势，这加速了微生物的积累并增加了糖尿病个体患龋齿的风险。
