Abstract：
OBJECTIVE: To develop a system for the culture of murine preantral ovarian follicles using Human Serum Albumin (HSA) and Human Platelet Lysate (PLTMax).
METHODS: Mechanically isolated preantral follicles (N=146) were obtained from Swiss mice and cultured in DMEM:F12 medium for ten days in a 96-well plate with conical bottom. The medium was supplemented with penicillin, streptomycin, and equine chorionic gonadotropin. Additional proteins were tested in 4 test groups: G1: human serum albumin (HSA), G2: human platelet lysate (PLTM), and G3 and G4: HSA + PLTMax at lower and higher concentrations, respectively. Cellular vitality and oocyte morphology were evaluated on day 11 of culture.
RESULTS: The highest follicular growth (3.4 fold) was achieved in HSA (G1), while a significantly lower (1.8 fold) growth was achieved in the presence of PLTM (G2, G4) and even further reduced (1.2 fold) when HSA and PLTM were combined (G3). Cellular vitality was close to 70-80% among the four groups, and the highest number of intact oocytes were found in G1.
CONCLUSIONS: PLTM did not improve follicular development and oocyte maturation compared to HSA but preserved cell vitality.
METHODS: Mechanically isolated preantral follicles (N=146) were obtained from Swiss mice and cultured in DMEM:F12 medium for ten days in a 96-well plate with conical bottom. The medium was supplemented with penicillin, streptomycin, and equine chorionic gonadotropin. Additional proteins were tested in 4 test groups: G1: human serum albumin (HSA), G2: human platelet lysate (PLTM), and G3 and G4: HSA + PLTMax at lower and higher concentrations, respectively. Cellular vitality and oocyte morphology were evaluated on day 11 of culture.
RESULTS: The highest follicular growth (3.4 fold) was achieved in HSA (G1), while a significantly lower (1.8 fold) growth was achieved in the presence of PLTM (G2, G4) and even further reduced (1.2 fold) when HSA and PLTM were combined (G3). Cellular vitality was close to 70-80% among the four groups, and the highest number of intact oocytes were found in G1.
CONCLUSIONS: PLTM did not improve follicular development and oocyte maturation compared to HSA but preserved cell vitality.
摘要：
目的：开发使用人血清白蛋白（HSA）和人血小板裂解物（PLTMax）培养鼠腔前卵巢卵泡的系统。
方法:从瑞士小鼠获得机械分离的腔前卵泡(N=146),并在具有锥形底部的96孔板中在DMEM:F12培养基中培养10天。培养基中补充了青霉素,链霉素,和马绒毛膜促性腺激素.在4个测试组中测试了其他蛋白质：G1：人血清白蛋白（HSA），G2：人血小板裂解物（PLTM），和G3和G4：HSA+PLTMax在较低和较高浓度，分别。在培养的第11天评估细胞活力和卵母细胞形态。
结果：在HSA（G1）中实现了最高的卵泡生长（3.4倍），而在PLTM（G2，G4）的存在下实现了显着较低的（1.8倍）生长，并且当HSA和PLTM组合（G3）时甚至进一步降低（1.2倍）。四组细胞活力接近70-80%,在G1中发现了最高数量的完整卵母细胞。
结论：与HSA相比，PLTM并未改善卵泡发育和卵母细胞成熟，但保留了细胞活力。
方法:从瑞士小鼠获得机械分离的腔前卵泡(N=146),并在具有锥形底部的96孔板中在DMEM:F12培养基中培养10天。培养基中补充了青霉素,链霉素,和马绒毛膜促性腺激素.在4个测试组中测试了其他蛋白质：G1：人血清白蛋白（HSA），G2：人血小板裂解物（PLTM），和G3和G4：HSA+PLTMax在较低和较高浓度，分别。在培养的第11天评估细胞活力和卵母细胞形态。
结果：在HSA（G1）中实现了最高的卵泡生长（3.4倍），而在PLTM（G2，G4）的存在下实现了显着较低的（1.8倍）生长，并且当HSA和PLTM组合（G3）时甚至进一步降低（1.2倍）。四组细胞活力接近70-80%,在G1中发现了最高数量的完整卵母细胞。
结论：与HSA相比，PLTM并未改善卵泡发育和卵母细胞成熟，但保留了细胞活力。
