Abstract：
Photothermal treatment (PTT) has emerged as a promising avenue for biofilm elimination, yet its potential drawbacks, such as local hyperpyrexia and bacterial heat resistance, have posed challenges. To address these concerns, an innovative nanoplatform (Au@mSiO2 -arg/ICG) is devised that integrates phototherapeutic and gas therapeutic functionalities. This multifaceted nanoplatform is composed of mesoporous silica-coated Au nanorods (Au@mSiO2 ), supplemented with l-arginine (l-arg) and indocyanine green (ICG), and is engineered for mild temperature PTT aimed at biofilm eradication. Au@mSiO2 -arg/ICG nanoparticles (NPs) show excellent antibacterial effects through the generation of nitric oxide (NO) gas, heat, and reactive oxygen species (ROS) under 808 nm light irradiation. The ROS generated by ICG initiates a cascade reaction with l-arg, ultimately yielding NO gas molecules. This localized release of NO not only effectively curbs the expression of heat shock proteins 70 mitigating bacterial thermoresistance, but also reduces extracellular polymeric substance allowing better penetration of the therapeutic agents. Furthermore, this nanoplatform achieves an outstanding biofilm elimination rate of over 99% in an abscess model under 808 nm light irradiation (0.8 W·cm-2 ), thereby establishing its potential as a dependable strategy for NO-enhanced mild PTT and antibacterial photodynamic therapy (aPDT) in clinical settings.
摘要：
光热处理（PTT）已成为消除生物膜的有希望的途径，然而它的潜在缺点，如局部高热和细菌耐热性，提出了挑战。为了解决这些问题，设计了一种创新的纳米平台（Au@mSiO2-arg/ICG），该平台集成了光疗和气体治疗功能。这种多面纳米平台由介孔二氧化硅涂覆的Au纳米棒（Au@mSiO2）组成，补充l-精氨酸（l-arg）和吲哚菁绿（ICG），并且被设计用于温和的温度PTT，旨在根除生物膜。Au@mSiO2-Arg/ICG纳米粒子(NPs)通过生成一氧化氮(NO)气体,热,和活性氧（ROS）在808nm的光照射下。ICG产生的ROS启动与l-arg的级联反应，最终产生NO气体分子。NO的这种局部释放不仅有效地抑制了热休克蛋白70的表达，减轻了细菌的耐热性。而且还减少了细胞外聚合物，从而使治疗剂更好地渗透。此外，该纳米平台在808nm光照射（0.8W·cm-2）下，在脓肿模型中实现了超过99％的出色生物膜消除率，从而确立了其作为临床环境中NO增强的轻度PTT和抗菌光动力疗法（aPDT）的可靠策略的潜力。
