UNASSIGNED: This study aimed to isolate high-affinity single-stranded DNA (ssDNA) aptamers against ETX.
UNASSIGNED: This study identified aptamers using the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) method, enzyme-linked apta-sorbent assay (ELASA), and surface plasmon resonance (SPR) to determine the affinity and specificity of the newly obtained aptamers targeting ETX.
UNASSIGNED: Several aptamers obtained through the SELEX process were studied. Among them, 2 aptamers, ETX clone 3 (ETX3; dissociation constant (Kd = 8.4 ± 2.4E-9M) and ETX11 (Kd = 6.3 ± 1.3E-9M) had favorable specificity for ETX. The limits of detection were 0.21 and 0.08 μg/mL for ETX3 and ETX11, respectively..
UNASSIGNED: The discovered aptamers can be used in various aptamer-based rapid diagnostic tests for the detection of ETX.
■本研究旨在分离抗ETX的高亲和力单链DNA(ssDNA)适体。
■这项研究使用指数富集(SELEX)方法对配体进行系统进化,酶联apta吸附剂测定(ELASA),和表面等离子体共振(SPR)来确定新获得的靶向ETX的适配体的亲和力和特异性。
■研究了通过SELEX方法获得的几种适体。其中,2个适体,ETX克隆3(ETX3;解离常数(Kd=8.4±2.4E-9M)和ETX11(Kd=6.3±1.3E-9M)对ETX具有良好的特异性。ETX3和ETX11的检出限分别为0.21和0.08μg/mL。.
■发现的适体可用于各种基于适体的快速诊断测试中,以检测ETX。