PDF(Pubmed)
Abstract：
Campylobacter is a major cause of bacterial foodborne diarrhea worldwide. Consumption of raw or undercooked chicken meat contaminated with Campylobacter is the most common causative agent of human infections. Given the high prevalence of contamination in poultry meat and the recent rise of multi-drug-resistant (MDR) Campylobacter strains, an effective intervention method of reducing bird colonization is needed. In this study, the Campylobacter-specific lytic phage CP6 was isolated from chicken feces. Phage CP6 exhibited a broad host range against different MDR Campylobacter isolates (97.4% of strains were infected). Some biological characteristics were observed, such as a good pH (3-9) stability and moderate temperature tolerance (<50 ℃). The complete genome sequence revealed a linear double-stranded DNA (178,350 bp, group II Campylobacter phage) with 27.51% GC content, including 209 predicted open reading frames, among which only 54 were annotated with known functions. Phylogenetic analysis of the phage major capsid protein demonstrated that phage CP6 was closely related to Campylobacter phage CPt10, CP21, CP20, IBB35, and CP220. CP6 phage exerted good antimicrobial effects on MDR Campylobacter in vitro culture and reduced CFUs of the host cells by up to 1-log compared with the control in artificially contaminated chicken breast meat. Our findings suggested the potential of CP6 phage as a promising antimicrobial agent for combating MDR Campylobacter in food processing.
摘要：
弯曲杆菌是世界范围内细菌性食源性腹泻的主要原因。食用受弯曲杆菌污染的生或未煮熟的鸡肉是人类感染的最常见病原体。鉴于禽肉污染的高发率和最近多重耐药（MDR）弯曲杆菌菌株的增加，需要一种减少鸟类定殖的有效干预方法。在这项研究中,从鸡粪便中分离出弯曲杆菌特异性裂解噬菌体CP6。噬菌体CP6对不同的MDR弯曲杆菌分离株表现出广泛的宿主范围(97.4%的菌株被感染)。观察到一些生物学特性，例如良好的pH（3-9）稳定性和中等温度耐受性（<50℃）。完整的基因组序列显示出线性双链DNA（178,350bp，II组弯曲杆菌噬菌体)，GC含量为27.51%，包括209个预测的开放阅读框架，其中只有54个注释了已知的函数。噬菌体主要衣壳蛋白的系统发育分析表明，噬菌体CP6与弯曲杆菌噬菌体CPt10，CP21，CP20，IBB35和CP220密切相关。CP6噬菌体对体外培养的MDR弯曲杆菌具有良好的抗菌作用，与人工污染的鸡胸肉中的对照相比，宿主细胞的CFU减少了1-log。我们的发现表明，CP6噬菌体作为一种有前途的抗微生物剂在食品加工中对抗MDR弯曲杆菌的潜力。
