Abstract：
Anaplasma phagocytophilum is a vector-borne zoonotic pathogen and can infect various vertebrate hosts, especially cattle, sheep, goats, horses, and dogs. Molecular-based studies have revealed that the agent has a high genetic diversity and closely related strains circulate in hosts. In this study, 618 sheep blood samples obtained from different geographic regions of Türkiye were researched for A.phagocytophilum and related strains with PCR, RFLP, and DNA sequence analyses. The DNA of these pathogens was detected in 110 (17.79%) samples. RFLP assay showed that all positive samples were infected with A.phagocytophilum-like 1, whereas A.phagocytophilum-like 2 and A.phagocytophilum were not detected. Partial parts of 16 S rRNA gene of seven randomly selected positive samples were sequenced. The phylogenetic analyses of these isolates revealed that at least two A.phagocytophilum-like 1 isolates circulate among hosts in Türkiye and around the world. A.phagocytophilum-related strains have been reported in molecular-based studies over the last few years, but there is a lack of data on the vector competence, epidemiology, clinical symptoms, and genetic diversity of these pathogens. Therefore, large-scale molecular studies are still needed to obtain detailed data on the above-mentioned topics.
摘要：
吞噬体是一种媒介传播的人畜共患病原体，可以感染多种脊椎动物宿主，尤其是牛,绵羊,山羊,马,还有狗.基于分子的研究表明，该试剂具有很高的遗传多样性，并且密切相关的菌株在宿主中循环。在这项研究中,用PCR方法研究了来自Türkiye不同地理区域的618份绵羊血样中的吞噬菌及其相关菌株，RFLP,和DNA序列分析。在110份(17.79%)样品中检测到这些病原体的DNA。RFLP分析显示，所有阳性样品均感染了吞噬细胞样1，而未检测到吞噬细胞样2和吞噬细胞样2。对7个随机选择的阳性样品的16SrRNA基因的部分部分进行测序。这些分离株的系统发育分析表明，至少有两个类似吞噬细胞1的分离株在Türkiye和世界各地的宿主之间循环。在过去的几年中，基于分子的研究已经报道了与吞噬细胞相关的菌株，但是缺乏关于矢量能力的数据，流行病学,临床症状,以及这些病原体的遗传多样性。因此,仍然需要大规模的分子研究来获得关于上述主题的详细数据。
