PDF(Pubmed)
Abstract：
In amniote limbs, Fibroblast Growth Factor 10 (FGF10) is essential for limb development, but whether this function is broadly conserved in tetrapods and/or involved in adult limb regeneration remains unknown. To tackle this question, we established Fgf10 mutant lines in the newt Pleurodeles waltl which has amazing regenerative ability. While Fgf10 mutant forelimbs develop normally, the hindlimbs fail to develop and downregulate FGF target genes. Despite these developmental defects, Fgf10 mutants were able to regenerate normal hindlimbs rather than recapitulating the embryonic phenotype. Together, our results demonstrate an important role for FGF10 in hindlimb formation, but little or no function in regeneration, suggesting that different mechanisms operate during limb regeneration versus development.
摘要：
在羊水的四肢，成纤维细胞生长因子10（FGF10）对肢体发育至关重要。但是这种功能是否在四足动物中广泛保守和/或是否参与成年肢体再生仍然未知。为了解决这个问题，我们在newpleurodeleswaltl中建立了Fgf10突变系，具有惊人的再生能力。虽然Fgf10突变前肢发育正常，后肢不能发育和下调FGF靶基因。尽管存在这些发育缺陷,Fgf10突变体能够再生正常的后肢，而不是重现胚胎表型。一起,我们的结果表明FGF10在后肢形成中的重要作用，但是再生功能很少或根本没有，这表明在肢体再生和发育过程中不同的机制起作用。
