Abstract：
D-Galactose (D-gal) accumulation triggers the generation of oxygen free radicals, resulting in skin aging. Sulforaphene (SFE), an isothiocyanate compound derived from radish seeds, possesses diverse biological activities, including protective effects against inflammation and oxidative damage. This investigation delves into the antioxidant impact of SFE on age-related skin injury. In vivo experiments demonstrate that SFE treatment significantly improves the macro- and micro-morphology of dorsal skin. It effectively diminishes the elevation of oxidative stress biomarkers in mice skin tissue treated with D-gal, concurrently enhancing the activity of antioxidant enzymes. Additionally, SFE mitigates collagen mRNA degradation, lowers pro-inflammatory cytokine levels, and downregulates MAPK-related protein expression in the skin. Moreover, SFE supplementation reduces lipid metabolite levels and elevates amino acid metabolites, such as L-cysteine and L-histidine. These findings suggest that SFE holds promise as a natural remedy to mitigate aging induced by oxidative stress.
摘要：
D-半乳糖（D-gal）积累引发氧自由基的产生，导致皮肤老化。莱芬(SFE),来自萝卜种子的异硫氰酸酯化合物，具有多种生物活性，包括对炎症和氧化损伤的保护作用。这项研究探讨了SFE对年龄相关皮肤损伤的抗氧化影响。体内实验表明，SFE治疗可显着改善背侧皮肤的宏观和微观形态。它有效地减少了用D-gal治疗的小鼠皮肤组织中氧化应激生物标志物的升高，同时增强抗氧化酶的活性。此外,SFE减轻胶原蛋白mRNA降解，降低促炎细胞因子水平，并下调皮肤中MAPK相关蛋白的表达。此外，补充SFE可降低脂质代谢产物水平并提高氨基酸代谢产物水平，例如L-半胱氨酸和L-组氨酸。这些发现表明，SFE有望作为缓解氧化应激引起的衰老的自然疗法。
