Abstract：
Aim: To study the expression of histone methyltransferase SMYD1 in white adipose tissue (WAT) and brown adipose tissue and during differentiation of preadipocytes to white and beige phenotypes. Methods: C57BL/6J mice fed a high-fat diet (and exposed to cold) and 3T3-L1 cells stimulated to differentiate into white and beige adipocytes were used. Results: SMYD1 expression increased in WAT of high-fat diet fed mice and in WAT and brown adipose tissue of cold-exposed mice, suggesting its role in thermogenesis. SMYD1 expression was higher in beige adipocytes than in white adipocytes, and its silencing leads to a decrease in mitochondrial content and in Pgc-1α expression. Conclusion: These data suggest a novel role for SMYD1 as a positive regulator of energy control in adipose tissue.
In this study, a protein called SMYD1 was examined in the adipose tissue of mice to understand its role in the development of different types of fat cells. The authors used mice fed a high-fat diet or mice exposed to a cold environment. The experiments were also performed on cultured cells that were stimulated to form specific types of fat cells (white adipocytes, which store energy; or beige adipocytes, which are responsible for releasing energy in the form of heat). The study found that SMYD1 increased in white adipose tissue particularly in response to cold exposure and high-fat diet, suggesting involvement in body temperature regulation. SMYD1 was higher in beige adipocytes than in white fat cells, and when SMYD1 was reduced, there was a decrease in certain factors related to energy control. Overall, these results suggest that SMYD1 plays a novel role in energy regulation in adipose tissues.
In this study, a protein called SMYD1 was examined in the adipose tissue of mice to understand its role in the development of different types of fat cells. The authors used mice fed a high-fat diet or mice exposed to a cold environment. The experiments were also performed on cultured cells that were stimulated to form specific types of fat cells (white adipocytes, which store energy; or beige adipocytes, which are responsible for releasing energy in the form of heat). The study found that SMYD1 increased in white adipose tissue particularly in response to cold exposure and high-fat diet, suggesting involvement in body temperature regulation. SMYD1 was higher in beige adipocytes than in white fat cells, and when SMYD1 was reduced, there was a decrease in certain factors related to energy control. Overall, these results suggest that SMYD1 plays a novel role in energy regulation in adipose tissues.
摘要：
目的：研究组蛋白甲基转移酶SMYD1在白色脂肪组织（WAT）和棕色脂肪组织中以及在前脂肪细胞向白色和米色表型分化过程中的表达。方法：使用高脂饮食（并暴露于寒冷中）的C57BL/6J小鼠和3T3-L1细胞刺激分化为白色和米色脂肪细胞。结果：SMYD1在高脂饮食小鼠的WAT和冷暴露小鼠的WAT和棕色脂肪组织中的表达增加，表明它在产热中的作用。SMYD1在米色脂肪细胞中的表达高于白色脂肪细胞，其沉默导致线粒体含量和Pgc-1α表达降低。结论：这些数据表明SMYD1作为脂肪组织能量控制的正调节剂的新作用。
在这项研究中,在小鼠的脂肪组织中检测了一种称为SMYD1的蛋白质，以了解其在不同类型脂肪细胞发育中的作用。作者使用高脂肪饮食的小鼠或暴露于寒冷环境的小鼠。实验还在培养的细胞上进行，这些细胞被刺激以形成特定类型的脂肪细胞（白色脂肪细胞，储存能量；或米色脂肪细胞，它们负责以热的形式释放能量)。研究发现，SMYD1在白色脂肪组织中增加，特别是在寒冷暴露和高脂肪饮食的反应中，提示参与体温调节。米色脂肪细胞的SMYD1高于白色脂肪细胞，当SMYD1减少时，与能量控制相关的某些因素有所减少。总的来说,这些结果表明，SMYD1在脂肪组织的能量调节中起着新的作用。
在这项研究中,在小鼠的脂肪组织中检测了一种称为SMYD1的蛋白质，以了解其在不同类型脂肪细胞发育中的作用。作者使用高脂肪饮食的小鼠或暴露于寒冷环境的小鼠。实验还在培养的细胞上进行，这些细胞被刺激以形成特定类型的脂肪细胞（白色脂肪细胞，储存能量；或米色脂肪细胞，它们负责以热的形式释放能量)。研究发现，SMYD1在白色脂肪组织中增加，特别是在寒冷暴露和高脂肪饮食的反应中，提示参与体温调节。米色脂肪细胞的SMYD1高于白色脂肪细胞，当SMYD1减少时，与能量控制相关的某些因素有所减少。总的来说,这些结果表明，SMYD1在脂肪组织的能量调节中起着新的作用。
