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Abstract：
Therapeutic research is very important in the prevention and treatment of leishmaniasis due to problems such as drug resistance, scarring and disease recurrence. The aim of this study was to determine how Leishmania major responds to the anti-leishmaniasis properties of podophyllotoxin and podophyllin. Cultured Leishmania promastigotes were exposed to different concentrations of podophyllotoxin and podophyllin for 24 and 48 h. Then, during the animal phase, Balb/c mice were experimentally injected with Leishmania promastigotes. After wounding, the effects of 0.5% podophyllotoxin and 25% podophyllin on reducing wound diameter and the number of amastigotes in the wound were evaluated. Podophyllotoxin and podophyllin were 83% and 59% lethal to Leishmania major promastigotes at the highest concentrations (200 µg/ml) and time (48 h). In the in vivo study, the mean lesion diameter at the end of treatment in the negative control group was 15.10 mm compared to 14.21 mm and 11.55 mm in the 25% podophyllin and 0.5% podophyllotoxin groups, respectively. Although both agents reduced the size of mice wounds and the number of amastigotes in the wounds, podophyllotoxin was more effective in this regard. Based on the results, podophyllotoxin and podophyllin can be used as leishmaniasis drugs after further research.
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摘要：
治疗研究在利什曼病的防治中具有十分重要的意义,疤痕和疾病复发。这项研究的目的是确定主要利什曼原虫如何响应鬼臼毒素和鬼臼素的抗利什曼病特性。将培养的利什曼原虫原虫暴露于不同浓度的鬼臼毒素和鬼臼素中24小时和48小时。然后，在动物阶段，Balb/c小鼠实验注射利什曼原虫。受伤后,评估了0.5％鬼臼毒素和25％鬼臼素对减少伤口直径和伤口中amastigotes数量的影响。在最高浓度（200µg/ml）和时间（48h）下，鬼臼毒素和鬼臼素对主要的利什曼原虫致死率分别为83％和59％。在体内研究中，阴性对照组治疗结束时的平均病变直径为15.10mm，而25％鬼臼毒素和0.5％鬼臼毒素组的平均病变直径为14.21mm和11.55mm，分别。尽管这两种药物都减少了小鼠伤口的大小和伤口中amastigotes的数量，鬼臼毒素在这方面更有效。根据结果,鬼臼毒素和鬼臼毒素经进一步研究后可作为利什曼病药物使用。
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