PDF(Pubmed)
Abstract：
The high incidence of oral carcinomas is due to its multifactorial etiology and the presence of various risk factors. Human Papillomavirus (HPV) has a proven role in the pathogenesis of oral carcinomas, but in the recent times there has been an increasing incidence of oral cancers who are negative for HPV infection. Also, these patients are non-smokers and non-drinkers so it could be speculated that these oral cancers are due to some other etiological factor probably of other viral infections. Therefore, this study examined the prevalence of Epstein Barr Virus (EBV) and Herpes Simplex Virus (HSV) among oral cancer patients. This cross-sectional study was conducted from January 2019 to June 2020. Biopsy samples from 47 newly diagnosed untreated patients with oral malignancies were collected along with their demographic and clinicopathological information. DNA extracted from the biopsies was processed for nested PCR for the detection of EBV and HSV. All the samples tested negative for HPV and HSV infection. Nested PCR detected 29 cases (70.7%) to be positive for EBV. The non-cancerous adjacent tissues also were negative for HPV, EBV and HSV. The prevalence of EBV was found to be more in males (62.1%) and the highest number of cases was of the left buccal mucosa compromising 34% of the total cases. From the present study it can be concluded that EBV but not HSV infection is associated with an increased risk of developing oral cancers. Although, 70.7% of the patients were found to be positive for EBV whether the viral infection played any role in the driving the malignancy needs to be further elucidated.
摘要：
口腔癌的高发病率是由于其多因素病因和各种危险因素的存在。人乳头瘤病毒（HPV）在口腔癌的发病机理中具有被证明的作用，但是最近，HPV感染阴性的口腔癌的发病率越来越高。此外，这些患者是非吸烟者和非饮酒者，因此可以推测这些口腔癌是由于其他一些病因，可能是其他病毒感染。因此,这项研究调查了口腔癌患者中EB病毒(EBV)和单纯疱疹病毒(HSV)的流行情况.这项横断面研究于2019年1月至2020年6月进行。收集了47名新诊断的未经治疗的口腔恶性肿瘤患者的活检样本以及他们的人口统计学和临床病理信息。处理从活检中提取的DNA用于巢式PCR以检测EBV和HSV。所有样品的HPV和HSV感染检测为阴性。巢式PCR检测29例(70.7%)EBV阳性。非癌邻近组织也是HPV阴性，EBV和HSV。发现男性中EBV的患病率更高（62.1％），病例数最高的是左颊粘膜，占总病例的34％。从本研究可以得出结论，EBV而非HSV感染与发展口腔癌的风险增加有关。虽然,发现70.7％的患者对EBV呈阳性，是否病毒感染在驱动恶性肿瘤中起任何作用需要进一步阐明。
