PDF(Pubmed)
Abstract：
BACKGROUND: Fatty acid-binding protein 4 (FABP4) has been associated with cardiovascular disease and diabetes. Acute aerobic exercise increases circulating FABP4 concentrations, but the underlying mechanisms remain unclear. The purpose of this study was to investigate the effects of inhibition of lipolysis by carbohydrate ingestion on circulating FABP4 concentrations during and after acute aerobic exercise in healthy men.
METHODS: Men aged between 20 and 40, with no exercise habits and no metabolic diseases, were recruited. In a randomized crossover design, the participants underwent a carbohydrate-ingestion exercise (CE) and a fasted exercise (FE) trial. The CE trial consisted of 40-min acute aerobic exercise with ingestion of carbohydrates and 60-min bed rest. The FE trial followed the same protocol as the CE trial but without carbohydrate ingestion. Venous blood samples were collected to measure hormones (adrenaline, noradrenaline, and insulin) metabolites (glycerol, free fatty acids, and glucose), and FABP4 concentrations. Ventilation and gas exchange were also collected to measure substrate oxidation.
RESULTS: Thirteen healthy men participated in and completed both the CE and FE trials. The insulin concentration was more than 4 times higher in the CE trial than in the FE trial (p < 0.004, effect size [ES] > 2.00). Free fatty acid concentrations were more than 4 times lower in the CE trial than in the FE trial (p < 0.02, ES > 2.04). However, there was no significant difference in the changes in circulating FABP4 concentrations between the CE and FE trials (p = 0.108), which did not change during aerobic exercise and significantly increased post-aerobic exercise in both trials (p < 0.002, ES > 1.212). Changes in FABP4 concentrations following aerobic exercise were not significantly correlated with changes in glycerol or free fatty acid concentrations during aerobic exercise.
CONCLUSIONS: The results suggest that suppression of lipolysis and elevation of insulin are not strongly involved in increases in FABP4 secretion following acute aerobic exercise.
METHODS: Men aged between 20 and 40, with no exercise habits and no metabolic diseases, were recruited. In a randomized crossover design, the participants underwent a carbohydrate-ingestion exercise (CE) and a fasted exercise (FE) trial. The CE trial consisted of 40-min acute aerobic exercise with ingestion of carbohydrates and 60-min bed rest. The FE trial followed the same protocol as the CE trial but without carbohydrate ingestion. Venous blood samples were collected to measure hormones (adrenaline, noradrenaline, and insulin) metabolites (glycerol, free fatty acids, and glucose), and FABP4 concentrations. Ventilation and gas exchange were also collected to measure substrate oxidation.
RESULTS: Thirteen healthy men participated in and completed both the CE and FE trials. The insulin concentration was more than 4 times higher in the CE trial than in the FE trial (p < 0.004, effect size [ES] > 2.00). Free fatty acid concentrations were more than 4 times lower in the CE trial than in the FE trial (p < 0.02, ES > 2.04). However, there was no significant difference in the changes in circulating FABP4 concentrations between the CE and FE trials (p = 0.108), which did not change during aerobic exercise and significantly increased post-aerobic exercise in both trials (p < 0.002, ES > 1.212). Changes in FABP4 concentrations following aerobic exercise were not significantly correlated with changes in glycerol or free fatty acid concentrations during aerobic exercise.
CONCLUSIONS: The results suggest that suppression of lipolysis and elevation of insulin are not strongly involved in increases in FABP4 secretion following acute aerobic exercise.
摘要：
背景：脂肪酸结合蛋白4（FABP4）与心血管疾病和糖尿病相关。急性有氧运动增加循环FABP4浓度，但潜在的机制仍不清楚。这项研究的目的是研究碳水化合物摄入抑制脂解对健康男性急性有氧运动期间和之后循环FABP4浓度的影响。
方法：20-40岁男性，无运动习惯，无代谢性疾病，被招募。在随机交叉设计中，参与者接受了碳水化合物摄入运动(CE)和禁食运动(FE)试验.CE试验包括40分钟的急性有氧运动，摄入碳水化合物和60分钟的卧床休息。FE试验遵循与CE试验相同的方案，但不摄入碳水化合物。收集静脉血样本以测量激素（肾上腺素，去甲肾上腺素,和胰岛素)代谢物(甘油，游离脂肪酸,和葡萄糖），和FABP4浓度。还收集通风和气体交换以测量底物氧化。
结果：13名健康男性参与并完成了CE和FE试验。CE试验中的胰岛素浓度比FE试验中的胰岛素浓度高4倍以上(p<0.004,效应大小[ES]>2.00)。CE试验中的游离脂肪酸浓度比FE试验中的游离脂肪酸浓度低4倍以上(p<0.02,ES>2.04)。然而,CE和FE试验之间循环FABP4浓度的变化没有显着差异（p=0.108），在两个试验中，有氧运动期间没有变化，并且在有氧运动后显着增加（p<0.002，ES>1.212）。有氧运动后FABP4浓度的变化与有氧运动期间甘油或游离脂肪酸浓度的变化没有显着相关。
结论：结果表明，急性有氧运动后，脂肪分解的抑制和胰岛素的升高与FABP4分泌的增加无关。
方法：20-40岁男性，无运动习惯，无代谢性疾病，被招募。在随机交叉设计中，参与者接受了碳水化合物摄入运动(CE)和禁食运动(FE)试验.CE试验包括40分钟的急性有氧运动，摄入碳水化合物和60分钟的卧床休息。FE试验遵循与CE试验相同的方案，但不摄入碳水化合物。收集静脉血样本以测量激素（肾上腺素，去甲肾上腺素,和胰岛素)代谢物(甘油，游离脂肪酸,和葡萄糖），和FABP4浓度。还收集通风和气体交换以测量底物氧化。
结果：13名健康男性参与并完成了CE和FE试验。CE试验中的胰岛素浓度比FE试验中的胰岛素浓度高4倍以上(p<0.004,效应大小[ES]>2.00)。CE试验中的游离脂肪酸浓度比FE试验中的游离脂肪酸浓度低4倍以上(p<0.02,ES>2.04)。然而,CE和FE试验之间循环FABP4浓度的变化没有显着差异（p=0.108），在两个试验中，有氧运动期间没有变化，并且在有氧运动后显着增加（p<0.002，ES>1.212）。有氧运动后FABP4浓度的变化与有氧运动期间甘油或游离脂肪酸浓度的变化没有显着相关。
结论：结果表明，急性有氧运动后，脂肪分解的抑制和胰岛素的升高与FABP4分泌的增加无关。
