Abstract:
This review summarizes the stepwise strategy and key points for magnetic beads (MBs)-based aptamer selection which is suitable for isolating aptamers against small and large molecules via systematic evolution of ligands by exponential enrichment (SELEX). Particularities, if any, are discussed according to the target size. Examples targeting small molecules (<1000 Da) such as xenobiotics, toxins, pesticides, herbicides, illegal additives, hormones, and large targets such as proteins (biomarkers, pathogens) are discussed and presented in tabular formats. Of special interest are the latest advances in more efficient alternatives, which are based on novel instrumentation, materials or microelectronics, such as fluorescence MBs-SELEX or microfluidic chip system-assisted MBs-SELEX. Limitations and perspectives of MBs-SELEX are also reviewed. Taken together, this review aims to provide practical insights into MBs-SELEX technologies and their ability to screen multiple potential aptamers against targets from small to large molecules.
摘要:
本文综述了基于磁珠(MBs)的适体选择的逐步策略和关键点,该方法适用于通过指数富集(SELEX)配体的系统进化来分离针对小分子和大分子的适体。特殊性,如果有的话,根据目标尺寸进行讨论。例如靶向小分子(<1000Da),如异种生物,毒素,杀虫剂,除草剂,非法添加剂,荷尔蒙,和大型靶标,如蛋白质(生物标志物,病原体)以表格形式进行讨论和呈现。特别令人感兴趣的是更有效替代品的最新进展,基于新颖的仪器,材料或微电子,例如荧光MBs-SELEX或微流控芯片系统辅助MBs-SELEX。还回顾了MBs-SELEX的局限性和观点。一起来看,本综述旨在为MBs-SELEX技术及其筛选多种潜在适体的能力提供实用见解。