PDF(Pubmed)
Abstract：
Tillering/branching pattern plays a significant role in determining the structure and diversity of grass, and trimming has been found to induce tillering in turfgrass. Recently, it has been reported that hydrogen peroxide (H2O2) regulates axillary bud development. However, the role of H2O2 in trimming-induced tillering in bermudagrass, a kind of turfgrass, remains unclear. Our study unveils the significant impact of trimming on promoting the sprouting and growth of tiller buds in stolon nodes, along with an increase in the number of tillers in the main stem. This effect is accompanied by spatial-temporal changes in cytokinin and sucrose content, as well as relevant gene expression in axillary buds. In addition, the partial trimming of new-born tillers results in an increase in sucrose and starch reserves in their leaves, which can be attributed to the enhanced photosynthesis capacity. Importantly, trimming promotes a rapid H2O2 burst in the leaves of new-born tillers and axillary stolon buds. Furthermore, exogenous application of H2O2 significantly increases the number of tillers after trimming by affecting the expression of cytokinin-related genes, bolstering photosynthesis potential, energy reserves and antioxidant enzyme activity. Taken together, these results indicate that both endogenous production and exogenous addition of H2O2 enhance the inductive effects of trimming on the tillering process in bermudagrass, thus helping boost energy supply and maintain the redox state in newly formed tillers.
摘要：
分枝/分枝模式在确定草的结构和多样性中起着重要作用，并且发现修剪会导致草坪草的分till。最近,据报道，过氧化氢（H2O2）调节腋芽的发育。然而,H2O2在百慕大草修剪诱导分枝中的作用，一种草皮草,尚不清楚。我们的研究揭示了修剪对促进茎节分茎芽发芽和生长的重要影响，随着主茎中分茎数量的增加。这种效应伴随着细胞分裂素和蔗糖含量的时空变化，以及相关基因在腋芽中的表达。此外,部分修剪新生分till会导致其叶片中蔗糖和淀粉储备的增加，这可以归因于光合作用能力的增强。重要的是,修剪可促进新生分till和腋生stolon芽的叶子中的H2O2快速爆发。此外，外源施用H2O2通过影响细胞分裂素相关基因的表达，显著增加修剪后的分ill数，增强光合作用潜力，能量储备和抗氧化酶活性。一起来看,这些结果表明，内源生产和外源添加H2O2都增强了修剪对百慕大分枝过程的诱导作用，从而帮助增加能量供应和维持氧化还原状态在新形成的分till。
