PDF(Pubmed)
Abstract：
Rice, a critical cereal crop, grapples with productivity challenges due to its inherent sensitivity to low temperatures, primarily during the seedling and booting stages. Recognizing the polygenic complexity of cold stress signaling in rice, a meta-analysis was undertaken, focusing on 20 physiological traits integral to cold tolerance. This initiative allowed the consolidation of genetic data from 242 QTLs into 58 meta-QTLs, thereby significantly constricting the genetic and physical intervals, with 84% of meta-QTLs (MQTLs) being reduced to less than 2 Mb. The list of 10,505 genes within these MQTLs, was further refined utilizing expression datasets to pinpoint 46 pivotal genes exhibiting noteworthy differential regulation during cold stress. The study underscored the presence of several TFs such as WRKY, NAC, CBF/DREB, MYB, and bHLH, known for their roles in cold stress response. Further, ortho-analysis involving maize, barley, and Arabidopsis identified OsWRKY71, among others, as a prospective candidate for enhancing cold tolerance in diverse crop plants. In conclusion, our study delineates the intricate genetic architecture underpinning cold tolerance in rice and propounds significant candidate genes, offering crucial insights for further research and breeding strategies focused on fortifying crops against cold stress, thereby bolstering global food resilience.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s12298-024-01412-1.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s12298-024-01412-1.
摘要：
大米,一种关键的谷类作物，由于其对低温的固有敏感性，应对生产力挑战，主要是在幼苗和孕穗期。认识到水稻冷胁迫信号的多基因复杂性，进行了荟萃分析，关注耐寒的20个生理性状。这一举措允许将242个QTL的遗传数据整合为58个元QTL，从而极大地限制了遗传和物理间隔，84%的meta-QTLs(MQTLs)降至2Mb以下。这些MQTL中的10,505个基因列表，利用表达数据集进一步完善，以查明在冷胁迫期间表现出值得注意的差异调节的46个关键基因。这项研究强调了几种TF的存在，如WRKY，NAC,CBF/DREB,MYB,和bHLH，以它们在寒冷应激反应中的作用而闻名。Further,涉及玉米的正交分析，大麦,拟南芥鉴定出OsWRKY71，作为增强多种作物耐寒性的潜在候选者。总之,我们的研究描绘了复杂的遗传结构支撑水稻耐寒性，并提出了重要的候选基因，为进一步的研究和育种策略提供了重要的见解，重点是加强作物对抗寒冷胁迫，从而增强全球粮食韧性。
■在线版本包含补充材料，可在10.1007/s12298-024-01412-1获得。
■在线版本包含补充材料，可在10.1007/s12298-024-01412-1获得。
